A genetic assay for gene essentiality in Clostridium
Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a sta...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/35788/ |
| _version_ | 1848795161296896000 |
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| author | Walker, David J.F. Heap, John T. Winzer, Klaus Minton, Nigel P. |
| author_facet | Walker, David J.F. Heap, John T. Winzer, Klaus Minton, Nigel P. |
| author_sort | Walker, David J.F. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene. |
| first_indexed | 2025-11-14T19:27:41Z |
| format | Article |
| id | nottingham-35788 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:27:41Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-357882020-05-04T18:23:52Z https://eprints.nottingham.ac.uk/35788/ A genetic assay for gene essentiality in Clostridium Walker, David J.F. Heap, John T. Winzer, Klaus Minton, Nigel P. Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene. Elsevier 2016-12-31 Article PeerReviewed Walker, David J.F., Heap, John T., Winzer, Klaus and Minton, Nigel P. (2016) A genetic assay for gene essentiality in Clostridium. Anaerobe, 42 . pp. 40-43. ISSN 1095-8274 Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target http://www.sciencedirect.com/science/article/pii/S1075996416300919 doi:10.1016/j.anaerobe.2016.07.007 doi:10.1016/j.anaerobe.2016.07.007 |
| spellingShingle | Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target Walker, David J.F. Heap, John T. Winzer, Klaus Minton, Nigel P. A genetic assay for gene essentiality in Clostridium |
| title | A genetic assay for gene essentiality in Clostridium |
| title_full | A genetic assay for gene essentiality in Clostridium |
| title_fullStr | A genetic assay for gene essentiality in Clostridium |
| title_full_unstemmed | A genetic assay for gene essentiality in Clostridium |
| title_short | A genetic assay for gene essentiality in Clostridium |
| title_sort | genetic assay for gene essentiality in clostridium |
| topic | Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target |
| url | https://eprints.nottingham.ac.uk/35788/ https://eprints.nottingham.ac.uk/35788/ https://eprints.nottingham.ac.uk/35788/ |