Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice

Physical exercise can improve cognition but whether this is related to motivation levels is unknown. Voluntary wheel running is a rewarding activity proposed as a model of motivation to exercise. To question the potential effects of exercise motivation on subsequent behaviour, we used a pharmacologi...

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Main Authors: Ebada, Mohamed Elsaed Elsayed, Kendall, David A., Pardon, Marie-Christine
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/35666/
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author Ebada, Mohamed Elsaed Elsayed
Kendall, David A.
Pardon, Marie-Christine
author_facet Ebada, Mohamed Elsaed Elsayed
Kendall, David A.
Pardon, Marie-Christine
author_sort Ebada, Mohamed Elsaed Elsayed
building Nottingham Research Data Repository
collection Online Access
description Physical exercise can improve cognition but whether this is related to motivation levels is unknown. Voluntary wheel running is a rewarding activity proposed as a model of motivation to exercise. To question the potential effects of exercise motivation on subsequent behaviour, we used a pharmacological approach targeting some reward mechanisms. The stress hormone corticosterone has rewarding effects mediated by activation of low affinity glucocorticoid receptors (GR). To investigate whether corticosterone synthesis motivates exercise via activation of GRs and subsequently, impacts on behaviour, we treated C57BL/6J mice acutely with the inhibitor of corticosterone synthesis metyrapone (35 mg/kg) or repeatedly with the GR antagonist mifepristone (30 mg/kg) prior to 1-h running wheel sessions. To investigate whether reducing motivation to exercise impacts on behaviour, we antagonised running-induced dopamine D2/D3 receptors activation with sulpiride (25 or 50 mg/kg) and assessed locomotor, anxietyrelated and memory performance after 20 running sessions over 4 weeks. We found that corticosterone synthesis contributes to running levels, but the maintenance of running behaviour was not mediated by activation of GRs. Intermittent exercise was not associated with changes in behavioural or cognitive performance. The persistent reduction in exercise levels triggered by sulpiride also had limited impact on behavioural performance, although the level of performance for some behaviours was related to the level of exercise. Altogether, these findings indicate that corticosterone and dopamine D2/D3 receptor activation contribute to the motivation for wheel running, but suggest that motivation for exercise is not a sufficient factor to alter behaviour in healthy mice.
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spelling nottingham-356662020-05-04T18:12:10Z https://eprints.nottingham.ac.uk/35666/ Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice Ebada, Mohamed Elsaed Elsayed Kendall, David A. Pardon, Marie-Christine Physical exercise can improve cognition but whether this is related to motivation levels is unknown. Voluntary wheel running is a rewarding activity proposed as a model of motivation to exercise. To question the potential effects of exercise motivation on subsequent behaviour, we used a pharmacological approach targeting some reward mechanisms. The stress hormone corticosterone has rewarding effects mediated by activation of low affinity glucocorticoid receptors (GR). To investigate whether corticosterone synthesis motivates exercise via activation of GRs and subsequently, impacts on behaviour, we treated C57BL/6J mice acutely with the inhibitor of corticosterone synthesis metyrapone (35 mg/kg) or repeatedly with the GR antagonist mifepristone (30 mg/kg) prior to 1-h running wheel sessions. To investigate whether reducing motivation to exercise impacts on behaviour, we antagonised running-induced dopamine D2/D3 receptors activation with sulpiride (25 or 50 mg/kg) and assessed locomotor, anxietyrelated and memory performance after 20 running sessions over 4 weeks. We found that corticosterone synthesis contributes to running levels, but the maintenance of running behaviour was not mediated by activation of GRs. Intermittent exercise was not associated with changes in behavioural or cognitive performance. The persistent reduction in exercise levels triggered by sulpiride also had limited impact on behavioural performance, although the level of performance for some behaviours was related to the level of exercise. Altogether, these findings indicate that corticosterone and dopamine D2/D3 receptor activation contribute to the motivation for wheel running, but suggest that motivation for exercise is not a sufficient factor to alter behaviour in healthy mice. Elsevier 2016-09-15 Article PeerReviewed Ebada, Mohamed Elsaed Elsayed, Kendall, David A. and Pardon, Marie-Christine (2016) Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice. Behavioural Brain Research, 311 . pp. 228-238. ISSN 1872-7549 Corticosterone; Voluntary wheel running; Dopamine D2/D3 receptors; Glucocorticoid receptors; Memory; C57BL/6J mice http://www.sciencedirect.com/science/article/pii/S0166432816303357 doi:10.1016/j.bbr.2016.05.051 doi:10.1016/j.bbr.2016.05.051
spellingShingle Corticosterone; Voluntary wheel running; Dopamine D2/D3 receptors; Glucocorticoid receptors; Memory; C57BL/6J mice
Ebada, Mohamed Elsaed Elsayed
Kendall, David A.
Pardon, Marie-Christine
Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title_full Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title_fullStr Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title_full_unstemmed Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title_short Corticosterone and dopamine D2/D3 receptors mediate the motivation for voluntary wheel running in C57BL/6J mice
title_sort corticosterone and dopamine d2/d3 receptors mediate the motivation for voluntary wheel running in c57bl/6j mice
topic Corticosterone; Voluntary wheel running; Dopamine D2/D3 receptors; Glucocorticoid receptors; Memory; C57BL/6J mice
url https://eprints.nottingham.ac.uk/35666/
https://eprints.nottingham.ac.uk/35666/
https://eprints.nottingham.ac.uk/35666/