Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka
Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
BioMed Central
2014
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/35412/ |
| _version_ | 1848795071175983104 |
|---|---|
| author | Gajapathy, Kanapathy Jude, Pavilupillai J. Goodacre, S.L. Peiris, Lalanthika B.S. Ramasamy, Ranjan Surendran, Sinnathamby N. |
| author_facet | Gajapathy, Kanapathy Jude, Pavilupillai J. Goodacre, S.L. Peiris, Lalanthika B.S. Ramasamy, Ranjan Surendran, Sinnathamby N. |
| author_sort | Gajapathy, Kanapathy |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.
Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.
Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand. |
| first_indexed | 2025-11-14T19:26:15Z |
| format | Article |
| id | nottingham-35412 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:26:15Z |
| publishDate | 2014 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-354122020-05-04T16:50:25Z https://eprints.nottingham.ac.uk/35412/ Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka Gajapathy, Kanapathy Jude, Pavilupillai J. Goodacre, S.L. Peiris, Lalanthika B.S. Ramasamy, Ranjan Surendran, Sinnathamby N. Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences. Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I. Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand. BioMed Central 2014-07-29 Article PeerReviewed Gajapathy, Kanapathy, Jude, Pavilupillai J., Goodacre, S.L., Peiris, Lalanthika B.S., Ramasamy, Ranjan and Surendran, Sinnathamby N. (2014) Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka. Parasites and Vectors, 7 (1). 348/1-348/5. ISSN 1756-3305 Anopheles barbirostris Malaria vector Species complex Sri Lanka http://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-348 doi:10.1186/1756-3305-7-348 doi:10.1186/1756-3305-7-348 |
| spellingShingle | Anopheles barbirostris Malaria vector Species complex Sri Lanka Gajapathy, Kanapathy Jude, Pavilupillai J. Goodacre, S.L. Peiris, Lalanthika B.S. Ramasamy, Ranjan Surendran, Sinnathamby N. Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title | Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title_full | Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title_fullStr | Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title_full_unstemmed | Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title_short | Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka |
| title_sort | molecular characterization of the malaria vector anopheles barbirostris van der wulp in sri lanka |
| topic | Anopheles barbirostris Malaria vector Species complex Sri Lanka |
| url | https://eprints.nottingham.ac.uk/35412/ https://eprints.nottingham.ac.uk/35412/ https://eprints.nottingham.ac.uk/35412/ |