Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach

Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars...

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Main Authors: Wilkinson, Stuart, Smart, Katherine A., James, Sue, Cook, David J.
Format: Article
Published: Springer 2017
Subjects:
Online Access:https://eprints.nottingham.ac.uk/35334/
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author Wilkinson, Stuart
Smart, Katherine A.
James, Sue
Cook, David J.
author_facet Wilkinson, Stuart
Smart, Katherine A.
James, Sue
Cook, David J.
author_sort Wilkinson, Stuart
building Nottingham Research Data Repository
collection Online Access
description Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A.oryzae and S.cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, one tonne of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A.oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.
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spelling nottingham-353342020-05-04T18:39:30Z https://eprints.nottingham.ac.uk/35334/ Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach Wilkinson, Stuart Smart, Katherine A. James, Sue Cook, David J. Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A.oryzae and S.cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, one tonne of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A.oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however it requires low energy and water inputs, and hence is worthy of further investigation and optimisation. Springer 2017-03-28 Article PeerReviewed Wilkinson, Stuart, Smart, Katherine A., James, Sue and Cook, David J. (2017) Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach. BioEnergy Research, 10 (1). pp. 146-157. ISSN 1939-1242 Brewers spent grains Lignocellulosic ethanol Consolidated bioprocessing Simultaneous saccharification and fermentation Brewery co-products Fungi http://link.springer.com/article/10.1007%2Fs12155-016-9782-7 doi:10.1007/s12155-016-9782-7 doi:10.1007/s12155-016-9782-7
spellingShingle Brewers spent grains
Lignocellulosic ethanol
Consolidated bioprocessing
Simultaneous saccharification and fermentation
Brewery co-products
Fungi
Wilkinson, Stuart
Smart, Katherine A.
James, Sue
Cook, David J.
Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title_full Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title_fullStr Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title_full_unstemmed Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title_short Bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (CBP) approach
title_sort bioethanol production from brewers spent grains using a fungal consolidated bioprocessing (cbp) approach
topic Brewers spent grains
Lignocellulosic ethanol
Consolidated bioprocessing
Simultaneous saccharification and fermentation
Brewery co-products
Fungi
url https://eprints.nottingham.ac.uk/35334/
https://eprints.nottingham.ac.uk/35334/
https://eprints.nottingham.ac.uk/35334/