Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse
Cytotoxic T-lymphocytes (CTLs) are associated with protective immunity against disease caused by equid herpesvirus type 1 (EHV-1). However, the EHV-1 target proteins for CTLs are poorly defined. This limits the development of vaccine candidates designed to stimulate strong CTL immunity. Here, classi...
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Microbiology Society
2014
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| Online Access: | https://eprints.nottingham.ac.uk/35230/ |
| _version_ | 1848795032406982656 |
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| author | Kydd, Julia H. Case, Ruth Minke, Julius Audonnet, Jean-Christophe Wagner, Bettina Antczak, Douglas F. |
| author_facet | Kydd, Julia H. Case, Ruth Minke, Julius Audonnet, Jean-Christophe Wagner, Bettina Antczak, Douglas F. |
| author_sort | Kydd, Julia H. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Cytotoxic T-lymphocytes (CTLs) are associated with protective immunity against disease caused by equid herpesvirus type 1 (EHV-1). However, the EHV-1 target proteins for CTLs are poorly defined. This limits the development of vaccine candidates designed to stimulate strong CTL immunity. Here, classical CTL assays using lymphocytes from horses of three defined MHC class I types that experienced natural infection with EHV-1 and a modified vaccinia virus construct containing an EHV-1 gene encoding the immediate-early (IE) protein are reported. Horses homozygous for the equine leukocyte antigen (ELA)-A2 haplotype, but not the ELA-A5 haplotype, produced MHC-restricted CTL responses against the IE protein. Previously, horses homozygous for the ELA-A3 haplotype also mounted CTL responses against the IE protein. Both haplotypes are common in major horse breeds, including the Thoroughbred. Thus, the IE protein is an attractive candidate molecule for future studies of T-cell immunity to EHV-1 in the horse. |
| first_indexed | 2025-11-14T19:25:38Z |
| format | Article |
| id | nottingham-35230 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:25:38Z |
| publishDate | 2014 |
| publisher | Microbiology Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-352302020-05-04T16:50:06Z https://eprints.nottingham.ac.uk/35230/ Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse Kydd, Julia H. Case, Ruth Minke, Julius Audonnet, Jean-Christophe Wagner, Bettina Antczak, Douglas F. Cytotoxic T-lymphocytes (CTLs) are associated with protective immunity against disease caused by equid herpesvirus type 1 (EHV-1). However, the EHV-1 target proteins for CTLs are poorly defined. This limits the development of vaccine candidates designed to stimulate strong CTL immunity. Here, classical CTL assays using lymphocytes from horses of three defined MHC class I types that experienced natural infection with EHV-1 and a modified vaccinia virus construct containing an EHV-1 gene encoding the immediate-early (IE) protein are reported. Horses homozygous for the equine leukocyte antigen (ELA)-A2 haplotype, but not the ELA-A5 haplotype, produced MHC-restricted CTL responses against the IE protein. Previously, horses homozygous for the ELA-A3 haplotype also mounted CTL responses against the IE protein. Both haplotypes are common in major horse breeds, including the Thoroughbred. Thus, the IE protein is an attractive candidate molecule for future studies of T-cell immunity to EHV-1 in the horse. Microbiology Society 2014-08-01 Article PeerReviewed Kydd, Julia H., Case, Ruth, Minke, Julius, Audonnet, Jean-Christophe, Wagner, Bettina and Antczak, Douglas F. (2014) Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse. Journal of General Virology, 95 . pp. 1783-1789. ISSN 1465-2099 Equine Herpesvirus Thoroughbred Cytotoxic T lymphocytes http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.065888-0 doi:10.1099/vir.0.065888-0 doi:10.1099/vir.0.065888-0 |
| spellingShingle | Equine Herpesvirus Thoroughbred Cytotoxic T lymphocytes Kydd, Julia H. Case, Ruth Minke, Julius Audonnet, Jean-Christophe Wagner, Bettina Antczak, Douglas F. Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title | Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title_full | Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title_fullStr | Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title_full_unstemmed | Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title_short | Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse |
| title_sort | immediate early protein of equid herpesvirus type 1 as a target for cytotoxic t-lymphocytes in the thoroughbred horse |
| topic | Equine Herpesvirus Thoroughbred Cytotoxic T lymphocytes |
| url | https://eprints.nottingham.ac.uk/35230/ https://eprints.nottingham.ac.uk/35230/ https://eprints.nottingham.ac.uk/35230/ |