Application of BRET to monitor ligand binding to GPCRs

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Application of BRET to monitor ligand binding to GPCRs

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligand...

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Bibliographic Details
Main Authors:	Stoddart, Leigh A., Johnstone, Elizabeth K.M., Wheal, Amanda J., Goulding, Joelle, Robers, Matthew B., Machleidt, Thomas, Wood, Keith V., Hill, Stephen J., Pfleger, Kevin D.G.
Format:	Article
Published:	Nature Publishing Group 2015
Subjects:	cell signalling drug development optical spectroscopy
Online Access:	https://eprints.nottingham.ac.uk/35176/

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