Application of BRET to monitor ligand binding to GPCRs

Application of BRET to monitor ligand binding to GPCRs

Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligand...

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Bibliographic Details
Main Authors: Stoddart, Leigh A., Johnstone, Elizabeth K.M., Wheal, Amanda J., Goulding, Joelle, Robers, Matthew B., Machleidt, Thomas, Wood, Keith V., Hill, Stephen J., Pfleger, Kevin D.G.
Format: Article
Published: Nature Publishing Group 2015
Subjects:
cell signalling
drug development
optical spectroscopy
Online Access:https://eprints.nottingham.ac.uk/35176/
Description
Summary:Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

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