Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training
We sought to ascertain the time course of transcriptional events that occur in human skeletal muscle at the outset of resistance exercise (RE) training in RE naive individuals and determine whether the magnitude of response was associated with exercise-induced muscle damage. Sixteen RE naive men wer...
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| Format: | Article |
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American Physiological Society
2014
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| Online Access: | https://eprints.nottingham.ac.uk/35099/ |
| _version_ | 1848795002427146240 |
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| author | Murton, Andrew J. Billeter, R. Stephens, Francis B. Des Etages, S.G. Graber, F. Hill, R.J. Marimuthu, Kanagaraj Greenhaff, Paul L. |
| author_facet | Murton, Andrew J. Billeter, R. Stephens, Francis B. Des Etages, S.G. Graber, F. Hill, R.J. Marimuthu, Kanagaraj Greenhaff, Paul L. |
| author_sort | Murton, Andrew J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We sought to ascertain the time course of transcriptional events that occur in human skeletal muscle at the outset of resistance exercise (RE) training in RE naive individuals and determine whether the magnitude of response was associated with exercise-induced muscle damage. Sixteen RE naive men were recruited; eight underwent two sessions of 5 × 30 maximum isokinetic knee extensions (180°/s) separated by 48 h. Muscle biopsies of the vastus lateralis, obtained from different sites, were taken at baseline and 24 h after each exercise bout. Eight individuals acted as nonexercise controls with biopsies obtained at the same time intervals. Transcriptional changes were assessed by microarray and protein levels of heat shock protein (HSP) 27 and αB-crystallin in muscle cross sections by immunohistochemistry as a proxy measure of muscle damage. In control subjects, no probe sets were significantly altered (false discovery rate < 0.05), and HSP27 and αB-crystallin protein remained unchanged throughout the study. In exercised subjects, significant intersubject variability following the initial RE bout was observed in the muscle transcriptome, with greatest changes occurring in subjects with elevated HSP27 and αB-crystallin protein. Following the second bout, the transcriptome response was more consistent, revealing a cohort of probe sets associated with immune activation, the suppression of oxidative metabolism, and ubiquitination, as differentially regulated. The results reveal that the initial transcriptional response to RE is variable in RE naive volunteers, potentially associated with muscle damage and unlikely to reflect longer term adaptations to RE training. These results highlight the importance of considering multiple time points when determining the transcriptional response to RE and associated physiological adaptation. |
| first_indexed | 2025-11-14T19:25:10Z |
| format | Article |
| id | nottingham-35099 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:25:10Z |
| publishDate | 2014 |
| publisher | American Physiological Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-350992020-05-04T16:40:20Z https://eprints.nottingham.ac.uk/35099/ Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training Murton, Andrew J. Billeter, R. Stephens, Francis B. Des Etages, S.G. Graber, F. Hill, R.J. Marimuthu, Kanagaraj Greenhaff, Paul L. We sought to ascertain the time course of transcriptional events that occur in human skeletal muscle at the outset of resistance exercise (RE) training in RE naive individuals and determine whether the magnitude of response was associated with exercise-induced muscle damage. Sixteen RE naive men were recruited; eight underwent two sessions of 5 × 30 maximum isokinetic knee extensions (180°/s) separated by 48 h. Muscle biopsies of the vastus lateralis, obtained from different sites, were taken at baseline and 24 h after each exercise bout. Eight individuals acted as nonexercise controls with biopsies obtained at the same time intervals. Transcriptional changes were assessed by microarray and protein levels of heat shock protein (HSP) 27 and αB-crystallin in muscle cross sections by immunohistochemistry as a proxy measure of muscle damage. In control subjects, no probe sets were significantly altered (false discovery rate < 0.05), and HSP27 and αB-crystallin protein remained unchanged throughout the study. In exercised subjects, significant intersubject variability following the initial RE bout was observed in the muscle transcriptome, with greatest changes occurring in subjects with elevated HSP27 and αB-crystallin protein. Following the second bout, the transcriptome response was more consistent, revealing a cohort of probe sets associated with immune activation, the suppression of oxidative metabolism, and ubiquitination, as differentially regulated. The results reveal that the initial transcriptional response to RE is variable in RE naive volunteers, potentially associated with muscle damage and unlikely to reflect longer term adaptations to RE training. These results highlight the importance of considering multiple time points when determining the transcriptional response to RE and associated physiological adaptation. American Physiological Society 2014-01-01 Article PeerReviewed Murton, Andrew J., Billeter, R., Stephens, Francis B., Des Etages, S.G., Graber, F., Hill, R.J., Marimuthu, Kanagaraj and Greenhaff, Paul L. (2014) Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training. Journal of Applied Physiology, 116 (1). pp. 2209-2214. ISSN 1522-1601 http://jap.physiology.org/content/116/1/113 doi:10.1152/japplphysiol.00426.2013 doi:10.1152/japplphysiol.00426.2013 |
| spellingShingle | Murton, Andrew J. Billeter, R. Stephens, Francis B. Des Etages, S.G. Graber, F. Hill, R.J. Marimuthu, Kanagaraj Greenhaff, Paul L. Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title | Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title_full | Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title_fullStr | Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title_full_unstemmed | Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title_short | Transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| title_sort | transient transcriptional events in human skeletal muscle at the outset of concentric resistance exercise training |
| url | https://eprints.nottingham.ac.uk/35099/ https://eprints.nottingham.ac.uk/35099/ https://eprints.nottingham.ac.uk/35099/ |