Mitotic activity of survivin is regulated by acetylation at K129
Survivin is a cancer-associated protein regulated by multiple factors, including acetylation at K129 within its C-terminal alpha-helical tail. Acetylation of survivin is being pursued as a potential prognostic marker in breast cancer. This modification at K129 may cause nuclear accumulation of surv...
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| Format: | Article |
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Taylor & Francis
2015
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| Online Access: | https://eprints.nottingham.ac.uk/35001/ |
| _version_ | 1848794979855499264 |
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| author | Aljaberi, Aysha M. Webster, Jamie R.M. Wheatley, Sally P. |
| author_facet | Aljaberi, Aysha M. Webster, Jamie R.M. Wheatley, Sally P. |
| author_sort | Aljaberi, Aysha M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Survivin is a cancer-associated protein regulated by multiple factors, including acetylation at K129 within its C-terminal alpha-helical tail. Acetylation of survivin is being pursued as a potential prognostic marker in breast cancer. This modification at K129 may cause nuclear accumulation of survivin in interphase cells; however, whether this affects its essential role during mitosis has not been addressed. We posited whether mimicking acetylation of survivin at K129 alters its activity during mitosis. Fluorescence microscopy and time-lapse imaging showed that, mutating this site to an alanine to act as a constitutive acetyl mimetic, K129A, causes defects in chromosome segregation and cytokinesis. As a non-acetylatable version, K129R, also has difficulty during mitotic exit, we conclude that cyclical acetylation and deacetylation is required for fully functional survivin during mitosis. |
| first_indexed | 2025-11-14T19:24:48Z |
| format | Article |
| id | nottingham-35001 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:24:48Z |
| publishDate | 2015 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-350012020-05-04T17:07:14Z https://eprints.nottingham.ac.uk/35001/ Mitotic activity of survivin is regulated by acetylation at K129 Aljaberi, Aysha M. Webster, Jamie R.M. Wheatley, Sally P. Survivin is a cancer-associated protein regulated by multiple factors, including acetylation at K129 within its C-terminal alpha-helical tail. Acetylation of survivin is being pursued as a potential prognostic marker in breast cancer. This modification at K129 may cause nuclear accumulation of survivin in interphase cells; however, whether this affects its essential role during mitosis has not been addressed. We posited whether mimicking acetylation of survivin at K129 alters its activity during mitosis. Fluorescence microscopy and time-lapse imaging showed that, mutating this site to an alanine to act as a constitutive acetyl mimetic, K129A, causes defects in chromosome segregation and cytokinesis. As a non-acetylatable version, K129R, also has difficulty during mitotic exit, we conclude that cyclical acetylation and deacetylation is required for fully functional survivin during mitosis. Taylor & Francis 2015-06-01 Article PeerReviewed Aljaberi, Aysha M., Webster, Jamie R.M. and Wheatley, Sally P. (2015) Mitotic activity of survivin is regulated by acetylation at K129. Cell Cycle, 14 (11). pp. 1738-1747. ISSN 1551-4005 apoptosis; acetylation; cancer; mitosis; survivin http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1033597 doi:10.1080/15384101.2015.1033597 doi:10.1080/15384101.2015.1033597 |
| spellingShingle | apoptosis; acetylation; cancer; mitosis; survivin Aljaberi, Aysha M. Webster, Jamie R.M. Wheatley, Sally P. Mitotic activity of survivin is regulated by acetylation at K129 |
| title | Mitotic activity of survivin is regulated by acetylation at K129 |
| title_full | Mitotic activity of survivin is regulated by acetylation at K129 |
| title_fullStr | Mitotic activity of survivin is regulated by acetylation at K129 |
| title_full_unstemmed | Mitotic activity of survivin is regulated by acetylation at K129 |
| title_short | Mitotic activity of survivin is regulated by acetylation at K129 |
| title_sort | mitotic activity of survivin is regulated by acetylation at k129 |
| topic | apoptosis; acetylation; cancer; mitosis; survivin |
| url | https://eprints.nottingham.ac.uk/35001/ https://eprints.nottingham.ac.uk/35001/ https://eprints.nottingham.ac.uk/35001/ |