Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity

Descending controls on spinal nociceptive processing play a pivotal role in shaping the pain experience following tissue injury. Secondary hypersensitivity develops within undamaged tissue adjacent, and distant to, damaged sites. Spinal neuronal pools innervating regions of secondary hypersensitivit...

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Main Authors: Drake, Robert A.R., Leith, J.L., Almahasneh, F., Martindale, J., Wilson, A.W., Lumb, B.M., Donaldson, Lucy F.
Format: Article
Published: Society for Neuroscience 2016
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Online Access:https://eprints.nottingham.ac.uk/34730/
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author Drake, Robert A.R.
Leith, J.L.
Almahasneh, F.
Martindale, J.
Wilson, A.W.
Lumb, B.M.
Donaldson, Lucy F.
author_facet Drake, Robert A.R.
Leith, J.L.
Almahasneh, F.
Martindale, J.
Wilson, A.W.
Lumb, B.M.
Donaldson, Lucy F.
author_sort Drake, Robert A.R.
building Nottingham Research Data Repository
collection Online Access
description Descending controls on spinal nociceptive processing play a pivotal role in shaping the pain experience following tissue injury. Secondary hypersensitivity develops within undamaged tissue adjacent, and distant to, damaged sites. Spinal neuronal pools innervating regions of secondary hypersensitivity are dominated by descending facilitation that amplifies spinal inputs from un-sensitized peripheral nociceptors. Cyclooxygenase–prostaglandin E2 signaling within the ventrolateral periaqueductal grey (vlPAG) is pro-nociceptive in naïve and acutely inflamed animals but its contributions in more prolonged inflammation and, importantly, secondary hypersensitivity remain unknown. In naïve rats, prostaglandin EP3 receptor (EP3R) antagonism in vlPAG modulated noxious withdrawal reflex (EMG) thresholds to preferential C-, but not A-, nociceptor activation, and raised thermal withdrawal thresholds in awake animals. In rats with inflammatory arthritis, secondary mechanical and thermal hypersensitivity of the hind-paw developed, and this was associated with spinal sensitization to Anociceptor inputs alone. In arthritic rats, blockade of vlPAG EP3R raised EMG thresholds to C-nociceptor activation in the area of secondary hypersensitivity to a degree equivalent to that evoked by the same manipulation in naïve rats.
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spelling nottingham-347302020-05-04T18:05:01Z https://eprints.nottingham.ac.uk/34730/ Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity Drake, Robert A.R. Leith, J.L. Almahasneh, F. Martindale, J. Wilson, A.W. Lumb, B.M. Donaldson, Lucy F. Descending controls on spinal nociceptive processing play a pivotal role in shaping the pain experience following tissue injury. Secondary hypersensitivity develops within undamaged tissue adjacent, and distant to, damaged sites. Spinal neuronal pools innervating regions of secondary hypersensitivity are dominated by descending facilitation that amplifies spinal inputs from un-sensitized peripheral nociceptors. Cyclooxygenase–prostaglandin E2 signaling within the ventrolateral periaqueductal grey (vlPAG) is pro-nociceptive in naïve and acutely inflamed animals but its contributions in more prolonged inflammation and, importantly, secondary hypersensitivity remain unknown. In naïve rats, prostaglandin EP3 receptor (EP3R) antagonism in vlPAG modulated noxious withdrawal reflex (EMG) thresholds to preferential C-, but not A-, nociceptor activation, and raised thermal withdrawal thresholds in awake animals. In rats with inflammatory arthritis, secondary mechanical and thermal hypersensitivity of the hind-paw developed, and this was associated with spinal sensitization to Anociceptor inputs alone. In arthritic rats, blockade of vlPAG EP3R raised EMG thresholds to C-nociceptor activation in the area of secondary hypersensitivity to a degree equivalent to that evoked by the same manipulation in naïve rats. Society for Neuroscience 2016-08-31 Article PeerReviewed Drake, Robert A.R., Leith, J.L., Almahasneh, F., Martindale, J., Wilson, A.W., Lumb, B.M. and Donaldson, Lucy F. (2016) Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity. Journal of Neuroscience, 36 (35). pp. 9026-9040. ISSN 1529-2401 Arthritis Descending Facilitation Inflammation Periaqueductal grey Prostaglandins Secondary Hyperalgesia http://www.jneurosci.org/content/36/35/9026 doi:10.1523/JNEUROSCI.4393-15.2016 doi:10.1523/JNEUROSCI.4393-15.2016
spellingShingle Arthritis
Descending Facilitation
Inflammation
Periaqueductal grey
Prostaglandins
Secondary Hyperalgesia
Drake, Robert A.R.
Leith, J.L.
Almahasneh, F.
Martindale, J.
Wilson, A.W.
Lumb, B.M.
Donaldson, Lucy F.
Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title_full Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title_fullStr Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title_full_unstemmed Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title_short Periaqueductal grey EP3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
title_sort periaqueductal grey ep3 receptors facilitate spinal nociception in arthritic secondary hypersensitivity
topic Arthritis
Descending Facilitation
Inflammation
Periaqueductal grey
Prostaglandins
Secondary Hyperalgesia
url https://eprints.nottingham.ac.uk/34730/
https://eprints.nottingham.ac.uk/34730/
https://eprints.nottingham.ac.uk/34730/