Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings
This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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American Chemical Society
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34683/ |
| _version_ | 1848794910967201792 |
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| author | Knopf-Marques, Helena Singh, Sonali Htwe, Su Su Wolfova, Lucie Buffa, Radovan Bacharouche, Jalal Francius, Grégory Voegel, Jean-Claude Schaaf, Pierre Ghaemmaghami, Amir M. Vrana, Nihal E. Lavalle, Philippe |
| author_facet | Knopf-Marques, Helena Singh, Sonali Htwe, Su Su Wolfova, Lucie Buffa, Radovan Bacharouche, Jalal Francius, Grégory Voegel, Jean-Claude Schaaf, Pierre Ghaemmaghami, Amir M. Vrana, Nihal E. Lavalle, Philippe |
| author_sort | Knopf-Marques, Helena |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of antiinflammatory cytokines (IL1-RA and CCL18) and the decrease of proinflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties. |
| first_indexed | 2025-11-14T19:23:42Z |
| format | Article |
| id | nottingham-34683 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:23:42Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-346832020-05-04T17:56:48Z https://eprints.nottingham.ac.uk/34683/ Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings Knopf-Marques, Helena Singh, Sonali Htwe, Su Su Wolfova, Lucie Buffa, Radovan Bacharouche, Jalal Francius, Grégory Voegel, Jean-Claude Schaaf, Pierre Ghaemmaghami, Amir M. Vrana, Nihal E. Lavalle, Philippe This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of antiinflammatory cytokines (IL1-RA and CCL18) and the decrease of proinflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties. American Chemical Society 2016-06-13 Article PeerReviewed Knopf-Marques, Helena, Singh, Sonali, Htwe, Su Su, Wolfova, Lucie, Buffa, Radovan, Bacharouche, Jalal, Francius, Grégory, Voegel, Jean-Claude, Schaaf, Pierre, Ghaemmaghami, Amir M., Vrana, Nihal E. and Lavalle, Philippe (2016) Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings. Biomacromolecules, 17 (6). pp. 2189-2198. ISSN 1525-7797 http://pubs.acs.org/doi/abs/10.1021/acs.biomac.6b00429 doi:10.1021/acs.biomac.6b00429 doi:10.1021/acs.biomac.6b00429 |
| spellingShingle | Knopf-Marques, Helena Singh, Sonali Htwe, Su Su Wolfova, Lucie Buffa, Radovan Bacharouche, Jalal Francius, Grégory Voegel, Jean-Claude Schaaf, Pierre Ghaemmaghami, Amir M. Vrana, Nihal E. Lavalle, Philippe Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title | Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title_full | Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title_fullStr | Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title_full_unstemmed | Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title_short | Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| title_sort | immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings |
| url | https://eprints.nottingham.ac.uk/34683/ https://eprints.nottingham.ac.uk/34683/ https://eprints.nottingham.ac.uk/34683/ |