Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth
We aimed to identify novel molecular mechanisms for muscle growth during administration of anabolic agents. Growing pigs (Duroc/(Landrace/Large-White)) were administered Ractopamine (a beta-adrenergic agonist; BA; 20 ppm in feed) or Reporcin (recombinant growth hormone; GH; 10 mg/48 hours injected)...
| Main Authors: | , , , , , , , , , , , , , |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34595/ |
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| author | Brown, D.M. Williams, H. Ryan, K.J.P. Wilson, T.L. Daniel, Zoe Mareko, M.H.D. Emes, Richard D. Harris, D.W. Jones, Sarah Wattis, Jonathan A.D. Dryden, Ian L. Hodgman, Charlie Brameld, John M. Parr, Tim |
| author_facet | Brown, D.M. Williams, H. Ryan, K.J.P. Wilson, T.L. Daniel, Zoe Mareko, M.H.D. Emes, Richard D. Harris, D.W. Jones, Sarah Wattis, Jonathan A.D. Dryden, Ian L. Hodgman, Charlie Brameld, John M. Parr, Tim |
| author_sort | Brown, D.M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We aimed to identify novel molecular mechanisms for muscle growth during administration of anabolic agents. Growing pigs (Duroc/(Landrace/Large-White)) were administered Ractopamine (a beta-adrenergic agonist; BA; 20 ppm in feed) or Reporcin (recombinant growth hormone; GH; 10 mg/48 hours injected) and compared to a control cohort (feed only; no injections) over a 27-day time course (1, 3, 7, 13 or 27-days). Longissimus Dorsi muscle gene expression was analyzed using Agilent porcine transcriptome microarrays and clusters of genes displaying similar expression profiles were identified using a modified maSigPro clustering algorithm. Anabolic agents increased carcass (p = 0.002) and muscle weights (Vastus Lateralis: p < 0.001; Semitendinosus: p = 0.075). Skeletal muscle mRNA expression of serine/one-carbon/glycine biosynthesis pathway genes (Phgdh, Psat1 and Psph) and the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase-M (Pck2/PEPCK-M), increased during treatment with BA, and to a lesser extent GH (p < 0.001, treatment x time interaction). Treatment with BA, but not GH, caused a 2-fold increase in phosphoglycerate dehydrogenase (PHGDH) protein expression at days 3 (p < 0.05) and 7 (p < 0.01), and a 2-fold increase in PEPCK-M protein expression at day 7 (p < 0.01). BA treated pigs exhibit a profound increase in expression of PHGDH and PEPCK-M in skeletal muscle, implicating a role for biosynthetic metabolic pathways in muscle growth. |
| first_indexed | 2025-11-14T19:23:22Z |
| format | Article |
| id | nottingham-34595 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:23:22Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-345952020-05-04T17:54:51Z https://eprints.nottingham.ac.uk/34595/ Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth Brown, D.M. Williams, H. Ryan, K.J.P. Wilson, T.L. Daniel, Zoe Mareko, M.H.D. Emes, Richard D. Harris, D.W. Jones, Sarah Wattis, Jonathan A.D. Dryden, Ian L. Hodgman, Charlie Brameld, John M. Parr, Tim We aimed to identify novel molecular mechanisms for muscle growth during administration of anabolic agents. Growing pigs (Duroc/(Landrace/Large-White)) were administered Ractopamine (a beta-adrenergic agonist; BA; 20 ppm in feed) or Reporcin (recombinant growth hormone; GH; 10 mg/48 hours injected) and compared to a control cohort (feed only; no injections) over a 27-day time course (1, 3, 7, 13 or 27-days). Longissimus Dorsi muscle gene expression was analyzed using Agilent porcine transcriptome microarrays and clusters of genes displaying similar expression profiles were identified using a modified maSigPro clustering algorithm. Anabolic agents increased carcass (p = 0.002) and muscle weights (Vastus Lateralis: p < 0.001; Semitendinosus: p = 0.075). Skeletal muscle mRNA expression of serine/one-carbon/glycine biosynthesis pathway genes (Phgdh, Psat1 and Psph) and the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase-M (Pck2/PEPCK-M), increased during treatment with BA, and to a lesser extent GH (p < 0.001, treatment x time interaction). Treatment with BA, but not GH, caused a 2-fold increase in phosphoglycerate dehydrogenase (PHGDH) protein expression at days 3 (p < 0.05) and 7 (p < 0.01), and a 2-fold increase in PEPCK-M protein expression at day 7 (p < 0.01). BA treated pigs exhibit a profound increase in expression of PHGDH and PEPCK-M in skeletal muscle, implicating a role for biosynthetic metabolic pathways in muscle growth. Nature Publishing Group 2016-06-28 Article PeerReviewed Brown, D.M., Williams, H., Ryan, K.J.P., Wilson, T.L., Daniel, Zoe, Mareko, M.H.D., Emes, Richard D., Harris, D.W., Jones, Sarah, Wattis, Jonathan A.D., Dryden, Ian L., Hodgman, Charlie, Brameld, John M. and Parr, Tim (2016) Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth. Scientific Reports, 6 . p. 28693. ISSN 2045-2322 Ractopamine Reporcin Anabolic Pigs Metabolism Muscle http://www.nature.com/articles/srep28693 doi:10.1038/srep28693 doi:10.1038/srep28693 |
| spellingShingle | Ractopamine Reporcin Anabolic Pigs Metabolism Muscle Brown, D.M. Williams, H. Ryan, K.J.P. Wilson, T.L. Daniel, Zoe Mareko, M.H.D. Emes, Richard D. Harris, D.W. Jones, Sarah Wattis, Jonathan A.D. Dryden, Ian L. Hodgman, Charlie Brameld, John M. Parr, Tim Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title | Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title_full | Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title_fullStr | Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title_full_unstemmed | Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title_short | Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| title_sort | mitochondrial phosphoenolpyruvate carboxykinase (pepck-m) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth |
| topic | Ractopamine Reporcin Anabolic Pigs Metabolism Muscle |
| url | https://eprints.nottingham.ac.uk/34595/ https://eprints.nottingham.ac.uk/34595/ https://eprints.nottingham.ac.uk/34595/ |