Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles
Novel magnetothermally responsive core-shell microparticles have been synthesized. The aqueous suspensions of these particles exhibit fast thermoreversible fluid-to-gel transitions and retain good magnetic properties. Rheological measurements demonstrated that the viscoelasticity of the prepared par...
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| Format: | Article |
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Royal Society of Chemistry
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34468/ |
| _version_ | 1848794860964806656 |
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| author | Braim, S. A. Shakesheff, Kevin M. Saunders, Brian R Alexander, Cameron |
| author_facet | Braim, S. A. Shakesheff, Kevin M. Saunders, Brian R Alexander, Cameron |
| author_sort | Braim, S. A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Novel magnetothermally responsive core-shell microparticles have been synthesized. The aqueous suspensions of these particles exhibit fast thermoreversible fluid-to-gel transitions and retain good magnetic properties. Rheological measurements demonstrated that the viscoelasticity of the prepared particle gels can be tuned, enabling these gels to have the mechanical properties that should facilitate their applications as 3D cell scaffolds for in vitro expansion of cells. Also, it was found that the responsive particles could be used in repeated heating-cooling cycles without marked changes in gel elasticity. Presto Blue viability assays of 3T3 fibroblasts and human mesenchymal sem cells cultured within the colloidal gel showed that the cells remained viable and proliferated, with significant increases in cell numbers over extended culture times. Confocal microscopy images of 3T3 cells cultured within the colloidal gel demonstrated that cells adhered, spread and retained their normal morphologies during proliferation.. Furthermore, magnetic separation allowed efficient recovery of cells after their expansion in vitro without need for enzyme-mediated release steps. Trypsin-free cell passages were performed allowing multiple growth, separation and reloading of cells within the colloidal gels. Overall, the results suggest this colloidal gel has potential as a 3D scaffold for in vitro expansion of a variety of cell types and for enzyme free cell harvesting. |
| first_indexed | 2025-11-14T19:22:55Z |
| format | Article |
| id | nottingham-34468 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:22:55Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-344682020-05-04T17:37:44Z https://eprints.nottingham.ac.uk/34468/ Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles Braim, S. A. Shakesheff, Kevin M. Saunders, Brian R Alexander, Cameron Novel magnetothermally responsive core-shell microparticles have been synthesized. The aqueous suspensions of these particles exhibit fast thermoreversible fluid-to-gel transitions and retain good magnetic properties. Rheological measurements demonstrated that the viscoelasticity of the prepared particle gels can be tuned, enabling these gels to have the mechanical properties that should facilitate their applications as 3D cell scaffolds for in vitro expansion of cells. Also, it was found that the responsive particles could be used in repeated heating-cooling cycles without marked changes in gel elasticity. Presto Blue viability assays of 3T3 fibroblasts and human mesenchymal sem cells cultured within the colloidal gel showed that the cells remained viable and proliferated, with significant increases in cell numbers over extended culture times. Confocal microscopy images of 3T3 cells cultured within the colloidal gel demonstrated that cells adhered, spread and retained their normal morphologies during proliferation.. Furthermore, magnetic separation allowed efficient recovery of cells after their expansion in vitro without need for enzyme-mediated release steps. Trypsin-free cell passages were performed allowing multiple growth, separation and reloading of cells within the colloidal gels. Overall, the results suggest this colloidal gel has potential as a 3D scaffold for in vitro expansion of a variety of cell types and for enzyme free cell harvesting. Royal Society of Chemistry 2016-02-07 Article PeerReviewed Braim, S. A., Shakesheff, Kevin M., Saunders, Brian R and Alexander, Cameron (2016) Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles. Journal of Materials Chemistry B, 4 (5). pp. 962-972. ISSN 2050-750X http://pubs.rsc.org/en/Content/ArticleLanding/2016/TB/C5TB01739D#!divAbstract doi:10.1039/c5tb01739d doi:10.1039/c5tb01739d |
| spellingShingle | Braim, S. A. Shakesheff, Kevin M. Saunders, Brian R Alexander, Cameron Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title | Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title_full | Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title_fullStr | Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title_full_unstemmed | Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title_short | Thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| title_sort | thermoresponsive magnetic colloidal gels via surface-initiated polymerisation from functional microparticles |
| url | https://eprints.nottingham.ac.uk/34468/ https://eprints.nottingham.ac.uk/34468/ https://eprints.nottingham.ac.uk/34468/ |