Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)

Objectives To address the hypothesis that different types of established OA pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low versus a high dose of...

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Main Authors: Nwosu, Lilian Ngozi, Mapp, Paul I., Chapman, Victoria, Walsh, David A.
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/34426/
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author Nwosu, Lilian Ngozi
Mapp, Paul I.
Chapman, Victoria
Walsh, David A.
author_facet Nwosu, Lilian Ngozi
Mapp, Paul I.
Chapman, Victoria
Walsh, David A.
author_sort Nwosu, Lilian Ngozi
building Nottingham Research Data Repository
collection Online Access
description Objectives To address the hypothesis that different types of established OA pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low versus a high dose of monosodium iodoacetate (MIA) in the rat. Methods Rats received a single intra-articular injection of 0.1mg or 1mg MIA or saline (control). Pain behaviour (hind limb weight bearing asymmetry (WB) and hindpaw withdrawal threshold (PWT) to punctate stimulation) was assessed. Cartilage and synovium were examined by macroscopic visualisation of articular surfaces and histopathology. Results Both doses of MIA lowered PWTs, 1mg MIA also resulted in WB asymmetry. Both doses were associated with cartilage macroscopic appearance, proteoglycan loss, abnormal chondrocyte morphology, increased numbers of vessels crossing the osteochondral junction, synovitis and macrophage infiltration into the synovium. PWTs were more strongly associated with chondrocyte morphology, synovitis and macrophage infiltration than with loss of cartilage surface integrity. Conclusions Both pain behaviours were associated with OA structural severity and synovitis. Differences in pain phenotype following low versus higher dose of MIA were identified despite similar structural pathology. OA structural pathology as traditionally measured only partially explains the MIA-induced pain phenotype.
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spelling nottingham-344262020-05-04T17:55:23Z https://eprints.nottingham.ac.uk/34426/ Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA) Nwosu, Lilian Ngozi Mapp, Paul I. Chapman, Victoria Walsh, David A. Objectives To address the hypothesis that different types of established OA pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low versus a high dose of monosodium iodoacetate (MIA) in the rat. Methods Rats received a single intra-articular injection of 0.1mg or 1mg MIA or saline (control). Pain behaviour (hind limb weight bearing asymmetry (WB) and hindpaw withdrawal threshold (PWT) to punctate stimulation) was assessed. Cartilage and synovium were examined by macroscopic visualisation of articular surfaces and histopathology. Results Both doses of MIA lowered PWTs, 1mg MIA also resulted in WB asymmetry. Both doses were associated with cartilage macroscopic appearance, proteoglycan loss, abnormal chondrocyte morphology, increased numbers of vessels crossing the osteochondral junction, synovitis and macrophage infiltration into the synovium. PWTs were more strongly associated with chondrocyte morphology, synovitis and macrophage infiltration than with loss of cartilage surface integrity. Conclusions Both pain behaviours were associated with OA structural severity and synovitis. Differences in pain phenotype following low versus higher dose of MIA were identified despite similar structural pathology. OA structural pathology as traditionally measured only partially explains the MIA-induced pain phenotype. Elsevier 2016-06-24 Article PeerReviewed Nwosu, Lilian Ngozi, Mapp, Paul I., Chapman, Victoria and Walsh, David A. (2016) Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA). Osteoarthritis and Cartilage . ISSN 1063-4584 (In Press) Pain behaviour; monosodium iodoacetate; synovitis; knee OA; structural pathology http://dx.doi.org/10.1016/j.joca.2016.06.012 10.1016/j.joca.2016.06.012 10.1016/j.joca.2016.06.012 10.1016/j.joca.2016.06.012
spellingShingle Pain behaviour; monosodium iodoacetate; synovitis; knee OA; structural pathology
Nwosu, Lilian Ngozi
Mapp, Paul I.
Chapman, Victoria
Walsh, David A.
Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title_full Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title_fullStr Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title_full_unstemmed Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title_short Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)
title_sort relationship between structural pathology and pain behaviour in a model of osteoarthritis (oa)
topic Pain behaviour; monosodium iodoacetate; synovitis; knee OA; structural pathology
url https://eprints.nottingham.ac.uk/34426/
https://eprints.nottingham.ac.uk/34426/
https://eprints.nottingham.ac.uk/34426/