Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures
Tanycytes play multiple roles in hypothalamic functions, including sensing peripheral nutrients and metabolic hormones, regulating neurosecretion and mediating seasonal cycles of reproduction and metabolic physiology. This last function reflects the expression of TSH receptors in tanycytes, which de...
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Bioscientifica
2015
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| Online Access: | https://eprints.nottingham.ac.uk/34340/ |
| _version_ | 1848794828548079616 |
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| author | Bolborea, Matei Helfer, Gisela Ebling, Francis J.P. Barrett, Perry |
| author_facet | Bolborea, Matei Helfer, Gisela Ebling, Francis J.P. Barrett, Perry |
| author_sort | Bolborea, Matei |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Tanycytes play multiple roles in hypothalamic functions, including sensing peripheral nutrients and metabolic hormones, regulating neurosecretion and mediating seasonal cycles of reproduction and metabolic physiology. This last function reflects the expression of TSH receptors in tanycytes, which detect photoperiod-regulated changes in TSH secretion from the neighbouring pars tuberalis. The present overall aim was to determine the signal transduction pathway by which TSH signals in tanycytes. Expression of the TSH receptor in tanycytes of 10-day-old Sprague Dawley rats was observed by in situ hybridisation. Primary ependymal cell cultures prepared from 10-day-old rats were found by immunohistochemistry to express vimentin but not GFAP and by PCR to express mRNA for Dio2, Gpr50, Darpp-32 and Tsh receptors that are characteristic of tanycytes. Treatment of primary tanycyte/ependymal cultures with TSH (100 IU/l) increased cAMP as assessed by ELISA and induced a cAMP-independent increase in the phosphorylation of ERK1/2 as assessed by western blot analysis. Furthermore, TSH (100 IU/l) stimulated a 2.17-fold increase in Dio2 mRNA expression. We conclude that TSH signal transduction in cultured tanycytes signals via Gαs to increase cAMP and via an alternative G protein to increase phosphorylation of ERK1/2. |
| first_indexed | 2025-11-14T19:22:24Z |
| format | Article |
| id | nottingham-34340 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:22:24Z |
| publishDate | 2015 |
| publisher | Bioscientifica |
| recordtype | eprints |
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| spelling | nottingham-343402020-05-04T17:07:16Z https://eprints.nottingham.ac.uk/34340/ Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures Bolborea, Matei Helfer, Gisela Ebling, Francis J.P. Barrett, Perry Tanycytes play multiple roles in hypothalamic functions, including sensing peripheral nutrients and metabolic hormones, regulating neurosecretion and mediating seasonal cycles of reproduction and metabolic physiology. This last function reflects the expression of TSH receptors in tanycytes, which detect photoperiod-regulated changes in TSH secretion from the neighbouring pars tuberalis. The present overall aim was to determine the signal transduction pathway by which TSH signals in tanycytes. Expression of the TSH receptor in tanycytes of 10-day-old Sprague Dawley rats was observed by in situ hybridisation. Primary ependymal cell cultures prepared from 10-day-old rats were found by immunohistochemistry to express vimentin but not GFAP and by PCR to express mRNA for Dio2, Gpr50, Darpp-32 and Tsh receptors that are characteristic of tanycytes. Treatment of primary tanycyte/ependymal cultures with TSH (100 IU/l) increased cAMP as assessed by ELISA and induced a cAMP-independent increase in the phosphorylation of ERK1/2 as assessed by western blot analysis. Furthermore, TSH (100 IU/l) stimulated a 2.17-fold increase in Dio2 mRNA expression. We conclude that TSH signal transduction in cultured tanycytes signals via Gαs to increase cAMP and via an alternative G protein to increase phosphorylation of ERK1/2. Bioscientifica 2015-06-01 Article PeerReviewed Bolborea, Matei, Helfer, Gisela, Ebling, Francis J.P. and Barrett, Perry (2015) Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures. Journal of Molecular Endocrinology, 54 . pp. 241-250. ISSN 1479-6813 Thyrotrophin Pars tuberalis Median eminence Neuroendocrine http://jme.endocrinology-journals.org/content/54/3/241 doi:10.1530/JME-14-0298 doi:10.1530/JME-14-0298 |
| spellingShingle | Thyrotrophin Pars tuberalis Median eminence Neuroendocrine Bolborea, Matei Helfer, Gisela Ebling, Francis J.P. Barrett, Perry Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title | Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title_full | Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title_fullStr | Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title_full_unstemmed | Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title_short | Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures |
| title_sort | dual signal transduction pathways activated by tsh receptors in rat primary tanycyte cultures |
| topic | Thyrotrophin Pars tuberalis Median eminence Neuroendocrine |
| url | https://eprints.nottingham.ac.uk/34340/ https://eprints.nottingham.ac.uk/34340/ https://eprints.nottingham.ac.uk/34340/ |