Technical considerations for the generation of novel pseudotyped viruses

A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the...

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Main Authors: King, Barnabas, Temperton, Nigel, Grehan, Keith, Scott, Simon D., Wright, Edward, Tarr, Alexander W., Daly, Janet M.
Format: Article
Published: Future Medicine 2016
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Online Access:https://eprints.nottingham.ac.uk/34242/
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author King, Barnabas
Temperton, Nigel
Grehan, Keith
Scott, Simon D.
Wright, Edward
Tarr, Alexander W.
Daly, Janet M.
author_facet King, Barnabas
Temperton, Nigel
Grehan, Keith
Scott, Simon D.
Wright, Edward
Tarr, Alexander W.
Daly, Janet M.
author_sort King, Barnabas
building Nottingham Research Data Repository
collection Online Access
description A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate pseudotyped viruses (PVs) with a focus on approaches to improve the efficacy of PV generation.
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spelling nottingham-342422020-05-04T20:04:36Z https://eprints.nottingham.ac.uk/34242/ Technical considerations for the generation of novel pseudotyped viruses King, Barnabas Temperton, Nigel Grehan, Keith Scott, Simon D. Wright, Edward Tarr, Alexander W. Daly, Janet M. A pseudotyped virus (PV) is a virus particle with an envelope protein originating from a different virus. The ability to dictate which envelope proteins are expressed on the surface has made pseudotyping an important tool for basic virological studies such as determining the cellular targets of the envelope protein as well as identification of potential antiviral compounds and measuring specific antibody responses. In this review, we describe the common methodologies employed to generate pseudotyped viruses (PVs) with a focus on approaches to improve the efficacy of PV generation. Future Medicine 2016-01 Article NonPeerReviewed King, Barnabas, Temperton, Nigel, Grehan, Keith, Scott, Simon D., Wright, Edward, Tarr, Alexander W. and Daly, Janet M. (2016) Technical considerations for the generation of novel pseudotyped viruses. Future Virology, 11 (1). pp. 47-59. ISSN 1746-0808 Pseudotyped virus pseudotypes pseudoparticles envelope glycoproteins http://www.futuremedicine.com/doi/10.2217/fvl.15.106 doi:10.2217/fvl.15.106 doi:10.2217/fvl.15.106
spellingShingle Pseudotyped virus
pseudotypes
pseudoparticles
envelope glycoproteins
King, Barnabas
Temperton, Nigel
Grehan, Keith
Scott, Simon D.
Wright, Edward
Tarr, Alexander W.
Daly, Janet M.
Technical considerations for the generation of novel pseudotyped viruses
title Technical considerations for the generation of novel pseudotyped viruses
title_full Technical considerations for the generation of novel pseudotyped viruses
title_fullStr Technical considerations for the generation of novel pseudotyped viruses
title_full_unstemmed Technical considerations for the generation of novel pseudotyped viruses
title_short Technical considerations for the generation of novel pseudotyped viruses
title_sort technical considerations for the generation of novel pseudotyped viruses
topic Pseudotyped virus
pseudotypes
pseudoparticles
envelope glycoproteins
url https://eprints.nottingham.ac.uk/34242/
https://eprints.nottingham.ac.uk/34242/
https://eprints.nottingham.ac.uk/34242/