Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment
In order to test therapeutics, functional assessments are required. In pre-clinical stroke research, there is little consensus regarding the most appropriate behavioural tasks to assess deficits; especially when testing over extended times in milder models with short occlusion times and small lesion...
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| Format: | Article |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34224/ |
| _version_ | 1848794801817780224 |
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| author | Trueman, Rebecca C. Diaz, Claris Farr, Tracy D. Harrison, David J. Fuller, Anna Tokarczuk, Paweł F. Stewart, Andrew J. Paisey, Stephen J. Dunnett, Stephen B. |
| author_facet | Trueman, Rebecca C. Diaz, Claris Farr, Tracy D. Harrison, David J. Fuller, Anna Tokarczuk, Paweł F. Stewart, Andrew J. Paisey, Stephen J. Dunnett, Stephen B. |
| author_sort | Trueman, Rebecca C. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In order to test therapeutics, functional assessments are required. In pre-clinical stroke research, there is little consensus regarding the most appropriate behavioural tasks to assess deficits; especially when testing over extended times in milder models with short occlusion times and small lesion volumes. In this study we comprehensively assessed 16 different behavioural tests, with the aim of identifying those that show robust, reliable and stable deficits for up to 2 months. These tasks are regularly used in stroke research, as well as being useful for examining striatal dysfunction in models of Huntington’s and Parkinson’s disease. Two cohorts of male Wistar rats underwent the intraluminal filament model of MCAO (30min) and were imaged 24hrs later. This resulted in primarily subcortical infarcts, with a small amount of cortical damage. Animals were tested, along with sham and naïve groups at 24hrs, 7 days, and 1 and 2 months. Following behavioural testing, brains were processed and striatal neuronal counts were performed alongside measurements of total brain and white matter atrophy. The staircase, adjusting steps, rotarod and apomorphine induced rotations were the most reliable for assessing long-term deficits in the 30 min transient MCAO model of stroke. |
| first_indexed | 2025-11-14T19:21:58Z |
| format | Article |
| id | nottingham-34224 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:21:58Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-342242020-05-04T17:56:12Z https://eprints.nottingham.ac.uk/34224/ Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment Trueman, Rebecca C. Diaz, Claris Farr, Tracy D. Harrison, David J. Fuller, Anna Tokarczuk, Paweł F. Stewart, Andrew J. Paisey, Stephen J. Dunnett, Stephen B. In order to test therapeutics, functional assessments are required. In pre-clinical stroke research, there is little consensus regarding the most appropriate behavioural tasks to assess deficits; especially when testing over extended times in milder models with short occlusion times and small lesion volumes. In this study we comprehensively assessed 16 different behavioural tests, with the aim of identifying those that show robust, reliable and stable deficits for up to 2 months. These tasks are regularly used in stroke research, as well as being useful for examining striatal dysfunction in models of Huntington’s and Parkinson’s disease. Two cohorts of male Wistar rats underwent the intraluminal filament model of MCAO (30min) and were imaged 24hrs later. This resulted in primarily subcortical infarcts, with a small amount of cortical damage. Animals were tested, along with sham and naïve groups at 24hrs, 7 days, and 1 and 2 months. Following behavioural testing, brains were processed and striatal neuronal counts were performed alongside measurements of total brain and white matter atrophy. The staircase, adjusting steps, rotarod and apomorphine induced rotations were the most reliable for assessing long-term deficits in the 30 min transient MCAO model of stroke. Nature Publishing Group 2016-06-17 Article PeerReviewed Trueman, Rebecca C., Diaz, Claris, Farr, Tracy D., Harrison, David J., Fuller, Anna, Tokarczuk, Paweł F., Stewart, Andrew J., Paisey, Stephen J. and Dunnett, Stephen B. (2016) Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment. Journal of Cerebral Blood Flow & Metabolism . ISSN 0271-678X Animal Models Behaviour (rodent) Brain Recovery Focal Ischemia Immunohistochemistry Stroke http://jcb.sagepub.com/content/early/2016/06/17/0271678X16654921 doi:10.1177/0271678X16654921 doi:10.1177/0271678X16654921 |
| spellingShingle | Animal Models Behaviour (rodent) Brain Recovery Focal Ischemia Immunohistochemistry Stroke Trueman, Rebecca C. Diaz, Claris Farr, Tracy D. Harrison, David J. Fuller, Anna Tokarczuk, Paweł F. Stewart, Andrew J. Paisey, Stephen J. Dunnett, Stephen B. Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title | Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title_full | Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title_fullStr | Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title_full_unstemmed | Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title_short | Systematic and detailed analysis of behavioural tests in the rat Middle Cerebral Artery Occlusion (MCAO) model of stroke: tests for long-term assessment |
| title_sort | systematic and detailed analysis of behavioural tests in the rat middle cerebral artery occlusion (mcao) model of stroke: tests for long-term assessment |
| topic | Animal Models Behaviour (rodent) Brain Recovery Focal Ischemia Immunohistochemistry Stroke |
| url | https://eprints.nottingham.ac.uk/34224/ https://eprints.nottingham.ac.uk/34224/ https://eprints.nottingham.ac.uk/34224/ |