SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance
Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Wiley
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34211/ |
| _version_ | 1848794799097774080 |
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| author | Asiani, Karishma R. Williams, Huw Edward Llewelyn Bird, Louise Jenner, Matthew Searle, Mark S. Hobman, Jon L. Scott, David J. Soultanas, Panos |
| author_facet | Asiani, Karishma R. Williams, Huw Edward Llewelyn Bird, Louise Jenner, Matthew Searle, Mark S. Hobman, Jon L. Scott, David J. Soultanas, Panos |
| author_sort | Asiani, Karishma R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions. |
| first_indexed | 2025-11-14T19:21:56Z |
| format | Article |
| id | nottingham-34211 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:21:56Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-342112020-05-04T17:52:42Z https://eprints.nottingham.ac.uk/34211/ SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance Asiani, Karishma R. Williams, Huw Edward Llewelyn Bird, Louise Jenner, Matthew Searle, Mark S. Hobman, Jon L. Scott, David J. Soultanas, Panos Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an ATPase (SilP), an effluxer complex (SilCFBA) and a periplasmic chaperon of Ag+ (SilE). SilE is predicted to be intrinsically disordered. We tested this hypothesis using structural and biophysical studies and show that SilE is an intrinsically disordered protein in its free apo-form but folds to a compact structure upon optimal binding to six Ag+ ions in its holo-form. Sequence analyses and site-directed mutagenesis established the importance of histidine and methionine containing motifs for Ag+-binding, and identified a nucleation core that initiates Ag+-mediated folding of SilE. We conclude that SilE is a molecular sponge for absorbing metal ions. Wiley 2016-05-07 Article PeerReviewed Asiani, Karishma R., Williams, Huw Edward Llewelyn, Bird, Louise, Jenner, Matthew, Searle, Mark S., Hobman, Jon L., Scott, David J. and Soultanas, Panos (2016) SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology . ISSN 1365-2958 http://onlinelibrary.wiley.com/doi/10.1111/mmi.13399/full doi:10.1111/mmi.13399 doi:10.1111/mmi.13399 |
| spellingShingle | Asiani, Karishma R. Williams, Huw Edward Llewelyn Bird, Louise Jenner, Matthew Searle, Mark S. Hobman, Jon L. Scott, David J. Soultanas, Panos SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title | SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title_full | SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title_fullStr | SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title_full_unstemmed | SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title_short | SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| title_sort | sile is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance |
| url | https://eprints.nottingham.ac.uk/34211/ https://eprints.nottingham.ac.uk/34211/ https://eprints.nottingham.ac.uk/34211/ |