Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging
We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Optical Society of America
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34189/ |
| _version_ | 1848794794408542208 |
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| author | Sinjab, Faris Kong, Kenny Gibson, Graham Varma, Sandeep Williams, Hywel C. Padgett, Miles Notingher, Ioan |
| author_facet | Sinjab, Faris Kong, Kenny Gibson, Graham Varma, Sandeep Williams, Hywel C. Padgett, Miles Notingher, Ioan |
| author_sort | Sinjab, Faris |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples. |
| first_indexed | 2025-11-14T19:21:51Z |
| format | Article |
| id | nottingham-34189 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:21:51Z |
| publishDate | 2016 |
| publisher | Optical Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-341892020-05-04T17:57:37Z https://eprints.nottingham.ac.uk/34189/ Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging Sinjab, Faris Kong, Kenny Gibson, Graham Varma, Sandeep Williams, Hywel C. Padgett, Miles Notingher, Ioan We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples. Optical Society of America 2016-06-06 Article PeerReviewed Sinjab, Faris, Kong, Kenny, Gibson, Graham, Varma, Sandeep, Williams, Hywel C., Padgett, Miles and Notingher, Ioan (2016) Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging. Biomedical Optics Express, 7 (8). pp. 2993-3006. ISSN 2156-7085 https://www.osapublishing.org/DirectPDFAccess/E1D19248-E17F-272F-4B783ED8BD54B1F4_345500/boe-7-8-2993.pdf?da=1&id=345500&seq=0&mobile=no doi:10.1364/BOE.7.002993 doi:10.1364/BOE.7.002993 |
| spellingShingle | Sinjab, Faris Kong, Kenny Gibson, Graham Varma, Sandeep Williams, Hywel C. Padgett, Miles Notingher, Ioan Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title | Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title_full | Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title_fullStr | Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title_full_unstemmed | Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title_short | Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging |
| title_sort | tissue diagnosis using power-sharing multifocal raman micro-spectroscopy and auto-fluorescence imaging |
| url | https://eprints.nottingham.ac.uk/34189/ https://eprints.nottingham.ac.uk/34189/ https://eprints.nottingham.ac.uk/34189/ |