Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessitate the availability of additional genetics tools...
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Oxford University Press
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34185/ |
| _version_ | 1848794793527738368 |
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| author | Zhang, Ying Xu, Shu Chai, Changsheng Yang, Sheng Jiang, Weihong Minton, Nigel P. Gu, Yang |
| author_facet | Zhang, Ying Xu, Shu Chai, Changsheng Yang, Sheng Jiang, Weihong Minton, Nigel P. Gu, Yang |
| author_sort | Zhang, Ying |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessitate the availability of additional genetics tools to facilitate the derivation of a greater understanding of the underlying metabolic and regulatory control processes in operation through forward genetic strategies. In this study, a xylose inducible, mariner-based, transposon system was developed and shown to allow high-efficient random mutagenesis in the model strain ATCC 824. Of the thiamphenicol resistant colonies obtained, 91.9% were shown to be due to successful transposition of the catP-based mini-transposon element. Phenotypic screening of 200 transposon clones revealed a sporulation-defective clone with an insertion in spo0A, thereby demonstrating that this inducible transposon system can be used for forward genetic studies in C. acetobutylicum. |
| first_indexed | 2025-11-14T19:21:50Z |
| format | Article |
| id | nottingham-34185 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:21:50Z |
| publishDate | 2016 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-341852020-05-04T17:44:29Z https://eprints.nottingham.ac.uk/34185/ Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum Zhang, Ying Xu, Shu Chai, Changsheng Yang, Sheng Jiang, Weihong Minton, Nigel P. Gu, Yang Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessitate the availability of additional genetics tools to facilitate the derivation of a greater understanding of the underlying metabolic and regulatory control processes in operation through forward genetic strategies. In this study, a xylose inducible, mariner-based, transposon system was developed and shown to allow high-efficient random mutagenesis in the model strain ATCC 824. Of the thiamphenicol resistant colonies obtained, 91.9% were shown to be due to successful transposition of the catP-based mini-transposon element. Phenotypic screening of 200 transposon clones revealed a sporulation-defective clone with an insertion in spo0A, thereby demonstrating that this inducible transposon system can be used for forward genetic studies in C. acetobutylicum. Oxford University Press 2016-04-30 Article PeerReviewed Zhang, Ying, Xu, Shu, Chai, Changsheng, Yang, Sheng, Jiang, Weihong, Minton, Nigel P. and Gu, Yang (2016) Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363 (8). fnw065/1-fnw065/7. ISSN 1574-6968 Clostridium; Transposon; Random mutagenesis; Xylose-inducible promoters https://academic.oup.com/femsle/article-lookup/doi/10.1093/femsle/fnw065 doi:10.1093/femsle/fnw065 doi:10.1093/femsle/fnw065 |
| spellingShingle | Clostridium; Transposon; Random mutagenesis; Xylose-inducible promoters Zhang, Ying Xu, Shu Chai, Changsheng Yang, Sheng Jiang, Weihong Minton, Nigel P. Gu, Yang Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title | Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title_full | Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title_fullStr | Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title_full_unstemmed | Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title_short | Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum |
| title_sort | development of an inducible transposon system for efficient random mutagenesis in clostridium acetobutylicum |
| topic | Clostridium; Transposon; Random mutagenesis; Xylose-inducible promoters |
| url | https://eprints.nottingham.ac.uk/34185/ https://eprints.nottingham.ac.uk/34185/ https://eprints.nottingham.ac.uk/34185/ |