An overview of technical considerations for Western blotting applications to physiological research
The applications of Western/immuno-blotting (WB) techniques have reached multiple layers of the scientific community and are now considered routine procedures in the field of physiology. This is none more so than in relation to skeletal muscle physiology (i.e. resolving the mechanisms underpinning a...
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Wiley
2016
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| Online Access: | https://eprints.nottingham.ac.uk/33950/ |
| _version_ | 1848794741641052160 |
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| author | Bass, Joseph J. Wilkinson, Daniel J. Rankin, Debbie Phillips, Bethan E. Szewczyk, Nathaniel J. Smith, Kenneth Atherton, Philip J. |
| author_facet | Bass, Joseph J. Wilkinson, Daniel J. Rankin, Debbie Phillips, Bethan E. Szewczyk, Nathaniel J. Smith, Kenneth Atherton, Philip J. |
| author_sort | Bass, Joseph J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The applications of Western/immuno-blotting (WB) techniques have reached multiple layers of the scientific community and are now considered routine procedures in the field of physiology. This is none more so than in relation to skeletal muscle physiology (i.e. resolving the mechanisms underpinning adaptations to exercise). Indeed, the inclusion of WB data is now considered an essential aspect of many such physiological publications to provide mechanistic insight into regulatory processes. Despite this popularity, and due to the ubiquitous and relatively inexpensive availability of WB equipment, the quality of WB in publications and subsequent analysis and interpretation of the data can be variable, perhaps resulting in spurious conclusions. This may be due to poor laboratory technique and/or lack of comprehension of the critical steps involved in WB and what quality control procedures should be in place to ensure robust data generation. The present review aims to provide a detailed description and critique of WB procedures and technicalities, from sample collection through preparation, blotting and detection to analysis of the data collected. We aim to provide the reader with improved expertise to critically conduct, evaluate and troubleshoot the WB process, to produce reproducible and reliable blots. |
| first_indexed | 2025-11-14T19:21:01Z |
| format | Article |
| id | nottingham-33950 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:21:01Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-339502020-05-04T17:57:38Z https://eprints.nottingham.ac.uk/33950/ An overview of technical considerations for Western blotting applications to physiological research Bass, Joseph J. Wilkinson, Daniel J. Rankin, Debbie Phillips, Bethan E. Szewczyk, Nathaniel J. Smith, Kenneth Atherton, Philip J. The applications of Western/immuno-blotting (WB) techniques have reached multiple layers of the scientific community and are now considered routine procedures in the field of physiology. This is none more so than in relation to skeletal muscle physiology (i.e. resolving the mechanisms underpinning adaptations to exercise). Indeed, the inclusion of WB data is now considered an essential aspect of many such physiological publications to provide mechanistic insight into regulatory processes. Despite this popularity, and due to the ubiquitous and relatively inexpensive availability of WB equipment, the quality of WB in publications and subsequent analysis and interpretation of the data can be variable, perhaps resulting in spurious conclusions. This may be due to poor laboratory technique and/or lack of comprehension of the critical steps involved in WB and what quality control procedures should be in place to ensure robust data generation. The present review aims to provide a detailed description and critique of WB procedures and technicalities, from sample collection through preparation, blotting and detection to analysis of the data collected. We aim to provide the reader with improved expertise to critically conduct, evaluate and troubleshoot the WB process, to produce reproducible and reliable blots. Wiley 2016-06-05 Article PeerReviewed Bass, Joseph J., Wilkinson, Daniel J., Rankin, Debbie, Phillips, Bethan E., Szewczyk, Nathaniel J., Smith, Kenneth and Atherton, Philip J. (2016) An overview of technical considerations for Western blotting applications to physiological research. Scandinavian Journal of Medicine & Science in Sports, 27 (1). pp. 4-25. ISSN 1600-0838 Westen blot physiology SDS PAGE skeletal muscle http://onlinelibrary.wiley.com/doi/10.1111/sms.12702/abstract doi:10.1111/sms.12702 doi:10.1111/sms.12702 |
| spellingShingle | Westen blot physiology SDS PAGE skeletal muscle Bass, Joseph J. Wilkinson, Daniel J. Rankin, Debbie Phillips, Bethan E. Szewczyk, Nathaniel J. Smith, Kenneth Atherton, Philip J. An overview of technical considerations for Western blotting applications to physiological research |
| title | An overview of technical considerations for Western blotting applications to physiological research |
| title_full | An overview of technical considerations for Western blotting applications to physiological research |
| title_fullStr | An overview of technical considerations for Western blotting applications to physiological research |
| title_full_unstemmed | An overview of technical considerations for Western blotting applications to physiological research |
| title_short | An overview of technical considerations for Western blotting applications to physiological research |
| title_sort | overview of technical considerations for western blotting applications to physiological research |
| topic | Westen blot physiology SDS PAGE skeletal muscle |
| url | https://eprints.nottingham.ac.uk/33950/ https://eprints.nottingham.ac.uk/33950/ https://eprints.nottingham.ac.uk/33950/ |