The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy
The calpains are a family of intracellular cysteine proteases that function in a variety of important cellular functions, including cell signalling, motility, apoptosis and survival. In early invasive breast cancer expression of calpain-1, calpain-2 and their inhibitor, calpastatin, have been associ...
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Impact Journals
2016
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| Online Access: | https://eprints.nottingham.ac.uk/33772/ |
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| author | Storr, Sarah J. Zhang, Siwei Perren, Tim Lansdown, Mark Fatayer, Hiba Sharma, Nisha Gahlaut, Renu Shaaban, Abeer Martin, Stewart G. |
| author_facet | Storr, Sarah J. Zhang, Siwei Perren, Tim Lansdown, Mark Fatayer, Hiba Sharma, Nisha Gahlaut, Renu Shaaban, Abeer Martin, Stewart G. |
| author_sort | Storr, Sarah J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The calpains are a family of intracellular cysteine proteases that function in a variety of important cellular functions, including cell signalling, motility, apoptosis and survival. In early invasive breast cancer expression of calpain-1, calpain-2 and their inhibitor, calpastatin, have been associated with clinical outcome and clinicopathological factors.
The expression of calpain-1, calpain-2 and calpastatin was determined using immunohistochemistry on core biopsy samples, in a cohort of large but operable inflammatory and non-inflammatory primary breast cancer patients treated with neoadjuvant chemotherapy. Information on treatment and prognostic variables together with long-term clinical follow-up was available for these patients. Diagnostic pre-chemotherapy core biopsy samples and surgically excised specimens were available for analysis.
Expression of calpastatin, calpain-1 or calpain-2 in the core biopsies was not associated with breast cancer specific survival in the total patient cohort; however, in patients with non-inflammatory breast cancer, high calpastatin expression was significantly associated with adverse breast cancer-specific survival (P=0.035), as was low calpain-2 expression (P=0.031). Low calpastatin expression was significantly associated with adverse breast cancer-specific survival of the inflammatory breast cancer patients (P=0.020), as was low calpain-1 expression (P=0.003).
In conclusion, high calpain-2 and low calpastatin expression is associated with improved breast cancer-specific survival in non-inflammatory large but operable primary breast cancer treated with neoadjuvant chemotherapy. In inflammatory cases, high calpain-1 and high calpastatin expression is associated with improved breast cancer-specific survival. Determining the expression of these proteins may be of clinical relevance. Further validation, in multi-centre cohorts of breast cancer patients treated with neoadjuvant chemotherapy, is warranted. |
| first_indexed | 2025-11-14T19:20:23Z |
| format | Article |
| id | nottingham-33772 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:20:23Z |
| publishDate | 2016 |
| publisher | Impact Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-337722020-05-04T17:56:36Z https://eprints.nottingham.ac.uk/33772/ The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy Storr, Sarah J. Zhang, Siwei Perren, Tim Lansdown, Mark Fatayer, Hiba Sharma, Nisha Gahlaut, Renu Shaaban, Abeer Martin, Stewart G. The calpains are a family of intracellular cysteine proteases that function in a variety of important cellular functions, including cell signalling, motility, apoptosis and survival. In early invasive breast cancer expression of calpain-1, calpain-2 and their inhibitor, calpastatin, have been associated with clinical outcome and clinicopathological factors. The expression of calpain-1, calpain-2 and calpastatin was determined using immunohistochemistry on core biopsy samples, in a cohort of large but operable inflammatory and non-inflammatory primary breast cancer patients treated with neoadjuvant chemotherapy. Information on treatment and prognostic variables together with long-term clinical follow-up was available for these patients. Diagnostic pre-chemotherapy core biopsy samples and surgically excised specimens were available for analysis. Expression of calpastatin, calpain-1 or calpain-2 in the core biopsies was not associated with breast cancer specific survival in the total patient cohort; however, in patients with non-inflammatory breast cancer, high calpastatin expression was significantly associated with adverse breast cancer-specific survival (P=0.035), as was low calpain-2 expression (P=0.031). Low calpastatin expression was significantly associated with adverse breast cancer-specific survival of the inflammatory breast cancer patients (P=0.020), as was low calpain-1 expression (P=0.003). In conclusion, high calpain-2 and low calpastatin expression is associated with improved breast cancer-specific survival in non-inflammatory large but operable primary breast cancer treated with neoadjuvant chemotherapy. In inflammatory cases, high calpain-1 and high calpastatin expression is associated with improved breast cancer-specific survival. Determining the expression of these proteins may be of clinical relevance. Further validation, in multi-centre cohorts of breast cancer patients treated with neoadjuvant chemotherapy, is warranted. Impact Journals 2016-06-15 Article PeerReviewed Storr, Sarah J., Zhang, Siwei, Perren, Tim, Lansdown, Mark, Fatayer, Hiba, Sharma, Nisha, Gahlaut, Renu, Shaaban, Abeer and Martin, Stewart G. (2016) The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy. Oncotarget . ISSN 1949-2553 (In Press) Calpain; Calpastatin; Breast cancer; Neoadjuvant chemotherapy; Survival http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=10066&author-preview=7rm doi:10.18632/oncotarget.10066 doi:10.18632/oncotarget.10066 |
| spellingShingle | Calpain; Calpastatin; Breast cancer; Neoadjuvant chemotherapy; Survival Storr, Sarah J. Zhang, Siwei Perren, Tim Lansdown, Mark Fatayer, Hiba Sharma, Nisha Gahlaut, Renu Shaaban, Abeer Martin, Stewart G. The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title | The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title_full | The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title_fullStr | The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title_full_unstemmed | The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title_short | The calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| title_sort | calpain system is associated with survival of breast cancer patients with large but operable inflammatory and non-inflammatory tumours treated with neoadjuvant chemotherapy |
| topic | Calpain; Calpastatin; Breast cancer; Neoadjuvant chemotherapy; Survival |
| url | https://eprints.nottingham.ac.uk/33772/ https://eprints.nottingham.ac.uk/33772/ https://eprints.nottingham.ac.uk/33772/ |