The role of copper(II) in the aggregation of human amylin
Amylin is the 37-residue peptide hormone produced by the islet β-cells in the pancreas and the formation of amylin aggregates is strongly associated with β-cells degeneration in type 2 diabetes, as demonstrated by more than 95% of patients exhibiting amylin amyloid upon autopsy. It is widely recogni...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2014
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| Online Access: | https://eprints.nottingham.ac.uk/3323/ |
| _version_ | 1848791002063568896 |
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| author | Sinopoli, Alessandro Magrì, Antonio Milardi, Danilo Pappalardo, Matteo Pucci, Pietro Flagiello, Angela Titman, Jeremy J. Nicoletti, Vincenzo G. Caruso, Giuseppe Pappalardo, Giuseppe Grasso, Giuseppe |
| author_facet | Sinopoli, Alessandro Magrì, Antonio Milardi, Danilo Pappalardo, Matteo Pucci, Pietro Flagiello, Angela Titman, Jeremy J. Nicoletti, Vincenzo G. Caruso, Giuseppe Pappalardo, Giuseppe Grasso, Giuseppe |
| author_sort | Sinopoli, Alessandro |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Amylin is the 37-residue peptide hormone produced by the islet β-cells in the pancreas and the formation of amylin aggregates is strongly associated with β-cells degeneration in type 2 diabetes, as demonstrated by more than 95% of patients exhibiting amylin amyloid upon autopsy. It is widely recognized that metal ions such as copper(II) have been implicated in the aggregation process of amyloidogenic peptides such as Aβ and α-synuclein and there is evidence that also amylin self-assembly is largely affected by copper(II). For this reason, in this work, the role of copper(II) in the aggregation of amylin has been investigated by several different experimental approaches. Mass spectrometric investigations show that copper(II) induces significant changes in the amylin structure which decrease the protein fibrillogenesis as observed by ThT measurements. Accordingly, solid-state NMR experiments together with computational analysis carried out on a model amylin fragment confirmed the non fibrillogenic nature of the copper(II) induced aggregated structure. Finally, the presence of copper(II) is also shown to have a major influence on amylin proneness to be degraded by proteases and cytotoxicity studies on different cell cultures are reported. |
| first_indexed | 2025-11-14T18:21:35Z |
| format | Article |
| id | nottingham-3323 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:21:35Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-33232020-05-04T16:53:05Z https://eprints.nottingham.ac.uk/3323/ The role of copper(II) in the aggregation of human amylin Sinopoli, Alessandro Magrì, Antonio Milardi, Danilo Pappalardo, Matteo Pucci, Pietro Flagiello, Angela Titman, Jeremy J. Nicoletti, Vincenzo G. Caruso, Giuseppe Pappalardo, Giuseppe Grasso, Giuseppe Amylin is the 37-residue peptide hormone produced by the islet β-cells in the pancreas and the formation of amylin aggregates is strongly associated with β-cells degeneration in type 2 diabetes, as demonstrated by more than 95% of patients exhibiting amylin amyloid upon autopsy. It is widely recognized that metal ions such as copper(II) have been implicated in the aggregation process of amyloidogenic peptides such as Aβ and α-synuclein and there is evidence that also amylin self-assembly is largely affected by copper(II). For this reason, in this work, the role of copper(II) in the aggregation of amylin has been investigated by several different experimental approaches. Mass spectrometric investigations show that copper(II) induces significant changes in the amylin structure which decrease the protein fibrillogenesis as observed by ThT measurements. Accordingly, solid-state NMR experiments together with computational analysis carried out on a model amylin fragment confirmed the non fibrillogenic nature of the copper(II) induced aggregated structure. Finally, the presence of copper(II) is also shown to have a major influence on amylin proneness to be degraded by proteases and cytotoxicity studies on different cell cultures are reported. Royal Society of Chemistry 2014-10-01 Article PeerReviewed Sinopoli, Alessandro, Magrì, Antonio, Milardi, Danilo, Pappalardo, Matteo, Pucci, Pietro, Flagiello, Angela, Titman, Jeremy J., Nicoletti, Vincenzo G., Caruso, Giuseppe, Pappalardo, Giuseppe and Grasso, Giuseppe (2014) The role of copper(II) in the aggregation of human amylin. Metallomics . ISSN 1756-5901 http://pubs.rsc.org/en/content/articlelanding/2014/mt/c4mt00130c#!divAbstract doi:10.1039/C4MT00130C doi:10.1039/C4MT00130C |
| spellingShingle | Sinopoli, Alessandro Magrì, Antonio Milardi, Danilo Pappalardo, Matteo Pucci, Pietro Flagiello, Angela Titman, Jeremy J. Nicoletti, Vincenzo G. Caruso, Giuseppe Pappalardo, Giuseppe Grasso, Giuseppe The role of copper(II) in the aggregation of human amylin |
| title | The role of copper(II) in the aggregation of human amylin |
| title_full | The role of copper(II) in the aggregation of human amylin |
| title_fullStr | The role of copper(II) in the aggregation of human amylin |
| title_full_unstemmed | The role of copper(II) in the aggregation of human amylin |
| title_short | The role of copper(II) in the aggregation of human amylin |
| title_sort | role of copper(ii) in the aggregation of human amylin |
| url | https://eprints.nottingham.ac.uk/3323/ https://eprints.nottingham.ac.uk/3323/ https://eprints.nottingham.ac.uk/3323/ |