Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men
Maximizing anabolic responses to feeding and exercise is crucial for muscle maintenance and adaptation to exercise training. We hypothesized that enriching a protein drink with leucine would improve anabolic responses to resistance exercise (RE: 6×8 knee-extension repetitions at 75% of 1-RM) in both...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/33108/ |
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| author | Atherton, Philip J. Kumar, Vinod Selby, Anna L. Rankin, Debbie Hildebrandt, Wulf Phillips, Bethan E. Williams, John P. Hiscock, Natalie Smith, Kenneth |
| author_facet | Atherton, Philip J. Kumar, Vinod Selby, Anna L. Rankin, Debbie Hildebrandt, Wulf Phillips, Bethan E. Williams, John P. Hiscock, Natalie Smith, Kenneth |
| author_sort | Atherton, Philip J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Maximizing anabolic responses to feeding and exercise is crucial for muscle maintenance and adaptation to exercise training. We hypothesized that enriching a protein drink with leucine would improve anabolic responses to resistance exercise (RE: 6×8 knee-extension repetitions at 75% of 1-RM) in both young and older adults. Groups (n=9) of young (24±6 y, BMI 23±2kg.m-2) and older men (70±5 y, BMI 25±2 kg.m-2) were randomized to either: (i) RE followed by Slim-Fast Optima (SFO 10 g PRO; 24 g CHO) with 4.2 g of leucine (LEU) or, (ii) RE+SFO with 4.2 g of alanine (ALA; isonitrogenous control). Muscle biopsies were taken before, immediately after, and 1, 2 and 4 h after RE and feeding. Muscle protein synthesis (MPS) was measured by incorporation of [1, 2-13C2] leucine into myofibrillar proteins and the phosphorylation of p70S6K1 by immunoblotting. In young men, both area under the curve (AUC; FSR 0-4 h P<0.05) and peak FSR (0.11 vs. 0.08%.h.-1; P<0.05) were greater in the SFO+LEU than in the SFO+ALA group, after RE. Similarly, in older men, AUC analysis revealed that post-exercise anabolic responses were greater in the SFO+LEU than SFO+ALA group, after RE (AUC; FSR 0-4 h P<0.05). Irrespective of age, increases in p70S6K1 phosphorylation were evident in response to both SFO+LEU and SFO+ALA, although greater with leucine supplementation than alanine (fold-change 2.2 vs. 3.2; P<0.05), specifically in the older men. We conclude that addition of Leucine to a sub-maximal PRO bolus improves anabolic responses to RE in young and older men. |
| first_indexed | 2025-11-14T19:18:06Z |
| format | Article |
| id | nottingham-33108 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:18:06Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-331082024-08-15T15:18:49Z https://eprints.nottingham.ac.uk/33108/ Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men Atherton, Philip J. Kumar, Vinod Selby, Anna L. Rankin, Debbie Hildebrandt, Wulf Phillips, Bethan E. Williams, John P. Hiscock, Natalie Smith, Kenneth Maximizing anabolic responses to feeding and exercise is crucial for muscle maintenance and adaptation to exercise training. We hypothesized that enriching a protein drink with leucine would improve anabolic responses to resistance exercise (RE: 6×8 knee-extension repetitions at 75% of 1-RM) in both young and older adults. Groups (n=9) of young (24±6 y, BMI 23±2kg.m-2) and older men (70±5 y, BMI 25±2 kg.m-2) were randomized to either: (i) RE followed by Slim-Fast Optima (SFO 10 g PRO; 24 g CHO) with 4.2 g of leucine (LEU) or, (ii) RE+SFO with 4.2 g of alanine (ALA; isonitrogenous control). Muscle biopsies were taken before, immediately after, and 1, 2 and 4 h after RE and feeding. Muscle protein synthesis (MPS) was measured by incorporation of [1, 2-13C2] leucine into myofibrillar proteins and the phosphorylation of p70S6K1 by immunoblotting. In young men, both area under the curve (AUC; FSR 0-4 h P<0.05) and peak FSR (0.11 vs. 0.08%.h.-1; P<0.05) were greater in the SFO+LEU than in the SFO+ALA group, after RE. Similarly, in older men, AUC analysis revealed that post-exercise anabolic responses were greater in the SFO+LEU than SFO+ALA group, after RE (AUC; FSR 0-4 h P<0.05). Irrespective of age, increases in p70S6K1 phosphorylation were evident in response to both SFO+LEU and SFO+ALA, although greater with leucine supplementation than alanine (fold-change 2.2 vs. 3.2; P<0.05), specifically in the older men. We conclude that addition of Leucine to a sub-maximal PRO bolus improves anabolic responses to RE in young and older men. Elsevier 2016-04-30 Article PeerReviewed Atherton, Philip J., Kumar, Vinod, Selby, Anna L., Rankin, Debbie, Hildebrandt, Wulf, Phillips, Bethan E., Williams, John P., Hiscock, Natalie and Smith, Kenneth (2016) Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men. Clinical Nutrition . ISSN 0261-5614 Muscle; protein; nutrition; exercise; aging http://dx.doi.org/10.1016/j.clnu.2016.04.025 doi:10.1016/j.clnu.2016.04.025 doi:10.1016/j.clnu.2016.04.025 |
| spellingShingle | Muscle; protein; nutrition; exercise; aging Atherton, Philip J. Kumar, Vinod Selby, Anna L. Rankin, Debbie Hildebrandt, Wulf Phillips, Bethan E. Williams, John P. Hiscock, Natalie Smith, Kenneth Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title | Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title_full | Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title_fullStr | Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title_full_unstemmed | Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title_short | Enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| title_sort | enriching a protein drink with leucine augments muscle protein synthesis after resistance exercise in young and older men |
| topic | Muscle; protein; nutrition; exercise; aging |
| url | https://eprints.nottingham.ac.uk/33108/ https://eprints.nottingham.ac.uk/33108/ https://eprints.nottingham.ac.uk/33108/ |