Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes

Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can interfere with bacterial adhesion or the chemical...

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Main Authors: Lui, Leong T., Xue, Xuan, Sui, Cheng, Brown, Alan, Pritchard, David I., Halliday, Nigel, Winzer, Klaus, Howdle, Stephen M., Fernandez-Trillo, Francisco, Krasnogor, Natalio, Alexander, Cameron
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Published: Nature Publishing Group 2013
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Online Access:https://eprints.nottingham.ac.uk/3302/
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author Lui, Leong T.
Xue, Xuan
Sui, Cheng
Brown, Alan
Pritchard, David I.
Halliday, Nigel
Winzer, Klaus
Howdle, Stephen M.
Fernandez-Trillo, Francisco
Krasnogor, Natalio
Alexander, Cameron
author_facet Lui, Leong T.
Xue, Xuan
Sui, Cheng
Brown, Alan
Pritchard, David I.
Halliday, Nigel
Winzer, Klaus
Howdle, Stephen M.
Fernandez-Trillo, Francisco
Krasnogor, Natalio
Alexander, Cameron
author_sort Lui, Leong T.
building Nottingham Research Data Repository
collection Online Access
description Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can interfere with bacterial adhesion or the chemical reactions used for quorum sensing are thus a potential means to control bacterial population responses. Here we report how polymeric "bacteria sequestrants", designed to bind to bacteria through electrostatic interactions and thus inhibit bacterial adhesion to surfaces, induce the expression of quorum sensing controlled phenotypes as a consequence of cell clustering. A combination of polymer and analytical chemistry, biological assays and computational modelling has been used to characterise the feedback between bacteria clustering and quorum sensing signaling. We have also derived design principles and chemical strategies for controlling bacterial behaviour at the population level
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institution University of Nottingham Malaysia Campus
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spelling nottingham-33022020-05-04T20:18:25Z https://eprints.nottingham.ac.uk/3302/ Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes Lui, Leong T. Xue, Xuan Sui, Cheng Brown, Alan Pritchard, David I. Halliday, Nigel Winzer, Klaus Howdle, Stephen M. Fernandez-Trillo, Francisco Krasnogor, Natalio Alexander, Cameron Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can interfere with bacterial adhesion or the chemical reactions used for quorum sensing are thus a potential means to control bacterial population responses. Here we report how polymeric "bacteria sequestrants", designed to bind to bacteria through electrostatic interactions and thus inhibit bacterial adhesion to surfaces, induce the expression of quorum sensing controlled phenotypes as a consequence of cell clustering. A combination of polymer and analytical chemistry, biological assays and computational modelling has been used to characterise the feedback between bacteria clustering and quorum sensing signaling. We have also derived design principles and chemical strategies for controlling bacterial behaviour at the population level Nature Publishing Group 2013-12 Article PeerReviewed Lui, Leong T., Xue, Xuan, Sui, Cheng, Brown, Alan, Pritchard, David I., Halliday, Nigel, Winzer, Klaus, Howdle, Stephen M., Fernandez-Trillo, Francisco, Krasnogor, Natalio and Alexander, Cameron (2013) Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes. Nature Chemistry, 5 (12). pp. 1058-1065. ISSN 1755-4349 Polymers bacteria quorum sensing molecular recognition synthetic biology http://www.nature.com/nchem/journal/v5/n12/full/nchem.1793.html doi:10.1038/nchem.1793 doi:10.1038/nchem.1793
spellingShingle Polymers
bacteria
quorum sensing
molecular recognition
synthetic biology
Lui, Leong T.
Xue, Xuan
Sui, Cheng
Brown, Alan
Pritchard, David I.
Halliday, Nigel
Winzer, Klaus
Howdle, Stephen M.
Fernandez-Trillo, Francisco
Krasnogor, Natalio
Alexander, Cameron
Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title_full Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title_fullStr Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title_full_unstemmed Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title_short Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
title_sort bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes
topic Polymers
bacteria
quorum sensing
molecular recognition
synthetic biology
url https://eprints.nottingham.ac.uk/3302/
https://eprints.nottingham.ac.uk/3302/
https://eprints.nottingham.ac.uk/3302/