Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting
Bone regeneration is a complex biological process where major cellular changes take place to support the osteogenic differentiation of mesenchymal bone progenitors. To characterise these biological changes and better understand the pathways regulating the formation of mature bone cells, the metaboli...
| Main Authors: | , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2016
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| Online Access: | https://eprints.nottingham.ac.uk/33015/ |
| _version_ | 1848794540793659392 |
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| author | Surrati, Amal Linforth, Rob S.T. Fisk, Ian D. Sottile, Virginie Kim, Dong-Hyun |
| author_facet | Surrati, Amal Linforth, Rob S.T. Fisk, Ian D. Sottile, Virginie Kim, Dong-Hyun |
| author_sort | Surrati, Amal |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Bone regeneration is a complex biological process where major cellular changes take place to support the osteogenic differentiation of mesenchymal bone progenitors. To characterise these biological changes and better understand the pathways regulating the formation of mature bone cells, the metabolic profile of mesenchymal stem cell (MSC) differentiation in vitro has been assessed non-invasively during osteogenic (OS) treatment using a footprinting technique. Liquid chromatography (LC)-mass spectrometry (MS)-based metabolite profiling of the culture medium was carried out in parallel to mineral deposition and alkaline phosphatase activity which are two hallmarks of osteogenesis in vitro. Metabolic profiles of spent culture media with a combination of univariate and multivariate analyses investigated concentration changes of extracellular metabolites and nutrients linked to the presence of MSCs in culture media. This non-invasive LC-MS-based analytical approach revealed significant metabolic changes between the media from control and OS-treated cells showing distinct effects of MSC differentiation on the environmental footprint of the cells in different conditions (control vs. OS treatment). A
subset of compounds was directly linked to the osteogenic time-course of differentiation, and represent interesting metabolite candidates as non-invasive biomarkers for characterising the differentiation of MSCs in a culture medium. |
| first_indexed | 2025-11-14T19:17:49Z |
| format | Article |
| id | nottingham-33015 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:17:49Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-330152020-05-04T17:46:10Z https://eprints.nottingham.ac.uk/33015/ Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting Surrati, Amal Linforth, Rob S.T. Fisk, Ian D. Sottile, Virginie Kim, Dong-Hyun Bone regeneration is a complex biological process where major cellular changes take place to support the osteogenic differentiation of mesenchymal bone progenitors. To characterise these biological changes and better understand the pathways regulating the formation of mature bone cells, the metabolic profile of mesenchymal stem cell (MSC) differentiation in vitro has been assessed non-invasively during osteogenic (OS) treatment using a footprinting technique. Liquid chromatography (LC)-mass spectrometry (MS)-based metabolite profiling of the culture medium was carried out in parallel to mineral deposition and alkaline phosphatase activity which are two hallmarks of osteogenesis in vitro. Metabolic profiles of spent culture media with a combination of univariate and multivariate analyses investigated concentration changes of extracellular metabolites and nutrients linked to the presence of MSCs in culture media. This non-invasive LC-MS-based analytical approach revealed significant metabolic changes between the media from control and OS-treated cells showing distinct effects of MSC differentiation on the environmental footprint of the cells in different conditions (control vs. OS treatment). A subset of compounds was directly linked to the osteogenic time-course of differentiation, and represent interesting metabolite candidates as non-invasive biomarkers for characterising the differentiation of MSCs in a culture medium. Royal Society of Chemistry 2016-04-18 Article PeerReviewed Surrati, Amal, Linforth, Rob S.T., Fisk, Ian D., Sottile, Virginie and Kim, Dong-Hyun (2016) Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting. Analyst, 12 . ISSN 1364-5528 http://pubs.rsc.org/en/Content/ArticleLanding/2016/AN/C6AN00170J#!divAbstract doi:10.1039/c6an00170j doi:10.1039/c6an00170j |
| spellingShingle | Surrati, Amal Linforth, Rob S.T. Fisk, Ian D. Sottile, Virginie Kim, Dong-Hyun Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title | Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title_full | Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title_fullStr | Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title_full_unstemmed | Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title_short | Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting |
| title_sort | non-destructive characterisation of mesenchymal stem cell differentiation using lc-ms-based metabolite footprinting |
| url | https://eprints.nottingham.ac.uk/33015/ https://eprints.nottingham.ac.uk/33015/ https://eprints.nottingham.ac.uk/33015/ |