Controlling equine influenza: traditional to next generation serological assays
Serological assays provide an indirect route for the recognition of infectious agents via the detection of antibodies against the infectious agent of interest within serum. Serological assays for equine influenza A virus can be applied for different purposes: diagnosing infections; subtyping isolate...
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| Format: | Article |
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Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/33013/ |
| _version_ | 1848794540123619328 |
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| author | Kinsley, Rebecca Scott, Simon D. Daly, Janet M. |
| author_facet | Kinsley, Rebecca Scott, Simon D. Daly, Janet M. |
| author_sort | Kinsley, Rebecca |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Serological assays provide an indirect route for the recognition of infectious agents via the detection of antibodies against the infectious agent of interest within serum. Serological assays for equine influenza A virus can be applied for different purposes: diagnosing infections; subtyping isolates; surveillance of circulating strains; and to evaluate the efficacy of vaccines before they reach the market. Haemagglutination inhibition (HI) and single radial haemolysis (SRH) assays are most commonly used in the equine field. This review outlines how both these assays together with virus neutralization (VN) and ELISA are performed, interpreted and applied for the control of equine influenza, giving the limitations and advantages of each. The pseudotyped virus neutralization assay (PVNA) is also discussed as a promising prospect for the future of equine influenza virus serology. |
| first_indexed | 2025-11-14T19:17:49Z |
| format | Article |
| id | nottingham-33013 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:17:49Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-330132020-05-04T17:43:37Z https://eprints.nottingham.ac.uk/33013/ Controlling equine influenza: traditional to next generation serological assays Kinsley, Rebecca Scott, Simon D. Daly, Janet M. Serological assays provide an indirect route for the recognition of infectious agents via the detection of antibodies against the infectious agent of interest within serum. Serological assays for equine influenza A virus can be applied for different purposes: diagnosing infections; subtyping isolates; surveillance of circulating strains; and to evaluate the efficacy of vaccines before they reach the market. Haemagglutination inhibition (HI) and single radial haemolysis (SRH) assays are most commonly used in the equine field. This review outlines how both these assays together with virus neutralization (VN) and ELISA are performed, interpreted and applied for the control of equine influenza, giving the limitations and advantages of each. The pseudotyped virus neutralization assay (PVNA) is also discussed as a promising prospect for the future of equine influenza virus serology. Elsevier 2016-05-01 Article PeerReviewed Kinsley, Rebecca, Scott, Simon D. and Daly, Janet M. (2016) Controlling equine influenza: traditional to next generation serological assays. Veterinary Microbiology, 187 . pp. 15-20. ISSN 0378-1135 Equine influenza ; Serological assay ; Neutralization assay http://www.sciencedirect.com/science/article/pii/S0378113516300591 doi:10.1016/j.vetmic.2016.03.006 doi:10.1016/j.vetmic.2016.03.006 |
| spellingShingle | Equine influenza ; Serological assay ; Neutralization assay Kinsley, Rebecca Scott, Simon D. Daly, Janet M. Controlling equine influenza: traditional to next generation serological assays |
| title | Controlling equine influenza: traditional to next generation serological assays |
| title_full | Controlling equine influenza: traditional to next generation serological assays |
| title_fullStr | Controlling equine influenza: traditional to next generation serological assays |
| title_full_unstemmed | Controlling equine influenza: traditional to next generation serological assays |
| title_short | Controlling equine influenza: traditional to next generation serological assays |
| title_sort | controlling equine influenza: traditional to next generation serological assays |
| topic | Equine influenza ; Serological assay ; Neutralization assay |
| url | https://eprints.nottingham.ac.uk/33013/ https://eprints.nottingham.ac.uk/33013/ https://eprints.nottingham.ac.uk/33013/ |