Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin

Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflamma...

Full description

Bibliographic Details
Main Authors: Farr, Tracy D., Lai, Chian-Hui, Grünstein, Dan, Orts-Gil, Guillermo, Wang, Cheng-Chung, Boehm-Sturm, Philipp, Seeberger, Peter H., Harms, Christoph
Format: Article
Published: American Chemical Society 2014
Subjects:
Online Access:https://eprints.nottingham.ac.uk/32797/
_version_ 1848794491823063040
author Farr, Tracy D.
Lai, Chian-Hui
Grünstein, Dan
Orts-Gil, Guillermo
Wang, Cheng-Chung
Boehm-Sturm, Philipp
Seeberger, Peter H.
Harms, Christoph
author_facet Farr, Tracy D.
Lai, Chian-Hui
Grünstein, Dan
Orts-Gil, Guillermo
Wang, Cheng-Chung
Boehm-Sturm, Philipp
Seeberger, Peter H.
Harms, Christoph
author_sort Farr, Tracy D.
building Nottingham Research Data Repository
collection Online Access
description Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflammatory proteins E and P selectin. Magnetic resonance imaging revealed that the targeted nanoparticles accumulated in the brain vasculature following acute administration into a clinically relevant animal model of stroke, though increases in selectin expression were observed in both brain hemispheres. Nonfunctionalized naked particles also appear to be a plausible agent to target the ischemic vasculature. The importance of these findings is discussed regarding the potential for translation into the clinic.
first_indexed 2025-11-14T19:17:03Z
format Article
id nottingham-32797
institution University of Nottingham Malaysia Campus
institution_category Local University
last_indexed 2025-11-14T19:17:03Z
publishDate 2014
publisher American Chemical Society
recordtype eprints
repository_type Digital Repository
spelling nottingham-327972020-05-04T16:42:49Z https://eprints.nottingham.ac.uk/32797/ Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin Farr, Tracy D. Lai, Chian-Hui Grünstein, Dan Orts-Gil, Guillermo Wang, Cheng-Chung Boehm-Sturm, Philipp Seeberger, Peter H. Harms, Christoph Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflammatory proteins E and P selectin. Magnetic resonance imaging revealed that the targeted nanoparticles accumulated in the brain vasculature following acute administration into a clinically relevant animal model of stroke, though increases in selectin expression were observed in both brain hemispheres. Nonfunctionalized naked particles also appear to be a plausible agent to target the ischemic vasculature. The importance of these findings is discussed regarding the potential for translation into the clinic. American Chemical Society 2014-02-25 Article PeerReviewed Farr, Tracy D., Lai, Chian-Hui, Grünstein, Dan, Orts-Gil, Guillermo, Wang, Cheng-Chung, Boehm-Sturm, Philipp, Seeberger, Peter H. and Harms, Christoph (2014) Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin. Nano Letters, 14 (4). pp. 2130-2134. ISSN 1530-6992 Glyco-magnetic nanoparticle; MRI; Stroke; Selection; Nano-medicine http://dx.doi.org/10.1021/nl500388h doi:10.1021/nl500388h doi:10.1021/nl500388h
spellingShingle Glyco-magnetic nanoparticle; MRI; Stroke; Selection; Nano-medicine
Farr, Tracy D.
Lai, Chian-Hui
Grünstein, Dan
Orts-Gil, Guillermo
Wang, Cheng-Chung
Boehm-Sturm, Philipp
Seeberger, Peter H.
Harms, Christoph
Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title_full Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title_fullStr Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title_full_unstemmed Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title_short Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
title_sort imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin
topic Glyco-magnetic nanoparticle; MRI; Stroke; Selection; Nano-medicine
url https://eprints.nottingham.ac.uk/32797/
https://eprints.nottingham.ac.uk/32797/
https://eprints.nottingham.ac.uk/32797/