Expression alterations define unique molecular characteristics of spinal ependymomas

Ependymomas are glial tumors that originate in either intracranial or spinal regions. Although tumors from different regions are histologically similar, they are biologically distinct. We therefore sought to identify molecular characteristics of spinal ependymomas (SEPN) in order to better understan...

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Main Authors: Lourdusamy, Anbarasu, Rahman, Ruman, Grundy, Richard G.
Format: Article
Published: Impact Journals 2015
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Online Access:https://eprints.nottingham.ac.uk/31769/
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author Lourdusamy, Anbarasu
Rahman, Ruman
Grundy, Richard G.
author_facet Lourdusamy, Anbarasu
Rahman, Ruman
Grundy, Richard G.
author_sort Lourdusamy, Anbarasu
building Nottingham Research Data Repository
collection Online Access
description Ependymomas are glial tumors that originate in either intracranial or spinal regions. Although tumors from different regions are histologically similar, they are biologically distinct. We therefore sought to identify molecular characteristics of spinal ependymomas (SEPN) in order to better understand the disease biology of these tumors. Using gene expression profiles of 256 tumor samples, we identified increased expression of 1,866 genes in SEPN when compared to intracranial ependymomas. These genes are mainly related to anterior/posterior pattern specification, response to oxidative stress, glial cell differentiation, DNA repair, and PPAR signalling, and also significantly enriched with cellular senescence genes (P = 5.5 × 10-03). In addition, a high number of significantly down-regulated genes in SEPN are localized to chromosome 22 (81 genes from chr22: 43,325,255 – 135,720,974; FDR = 1.77 × 10-23 and 22 genes from chr22: 324,739 – 32,822,302; FDR = 2.07 × 10-09) including BRD1, EP300, HDAC10, HIRA, HIC2, MKL1, and NF2. Evaluation of NF2 co-expressed genes further confirms the enrichment of chromosome 22 regions. Finally, systematic integration of chromosome 22 genes with interactome and NF2 co-expression data identifies key candidate genes. Our results reveal unique molecular characteristics of SEPN such as altered expression of cellular senescence and chromosome 22 genes.
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spelling nottingham-317692020-05-04T17:03:45Z https://eprints.nottingham.ac.uk/31769/ Expression alterations define unique molecular characteristics of spinal ependymomas Lourdusamy, Anbarasu Rahman, Ruman Grundy, Richard G. Ependymomas are glial tumors that originate in either intracranial or spinal regions. Although tumors from different regions are histologically similar, they are biologically distinct. We therefore sought to identify molecular characteristics of spinal ependymomas (SEPN) in order to better understand the disease biology of these tumors. Using gene expression profiles of 256 tumor samples, we identified increased expression of 1,866 genes in SEPN when compared to intracranial ependymomas. These genes are mainly related to anterior/posterior pattern specification, response to oxidative stress, glial cell differentiation, DNA repair, and PPAR signalling, and also significantly enriched with cellular senescence genes (P = 5.5 × 10-03). In addition, a high number of significantly down-regulated genes in SEPN are localized to chromosome 22 (81 genes from chr22: 43,325,255 – 135,720,974; FDR = 1.77 × 10-23 and 22 genes from chr22: 324,739 – 32,822,302; FDR = 2.07 × 10-09) including BRD1, EP300, HDAC10, HIRA, HIC2, MKL1, and NF2. Evaluation of NF2 co-expressed genes further confirms the enrichment of chromosome 22 regions. Finally, systematic integration of chromosome 22 genes with interactome and NF2 co-expression data identifies key candidate genes. Our results reveal unique molecular characteristics of SEPN such as altered expression of cellular senescence and chromosome 22 genes. Impact Journals 2015-03-30 Article PeerReviewed Lourdusamy, Anbarasu, Rahman, Ruman and Grundy, Richard G. (2015) Expression alterations define unique molecular characteristics of spinal ependymomas. Oncotarget, 6 (23). pp. 19780-19791. ISSN 1949-2553 ependymoma gene expression meta-analysis co-expression network http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=3715 doi:10.18632/oncotarget.3715 doi:10.18632/oncotarget.3715
spellingShingle ependymoma
gene expression
meta-analysis
co-expression network
Lourdusamy, Anbarasu
Rahman, Ruman
Grundy, Richard G.
Expression alterations define unique molecular characteristics of spinal ependymomas
title Expression alterations define unique molecular characteristics of spinal ependymomas
title_full Expression alterations define unique molecular characteristics of spinal ependymomas
title_fullStr Expression alterations define unique molecular characteristics of spinal ependymomas
title_full_unstemmed Expression alterations define unique molecular characteristics of spinal ependymomas
title_short Expression alterations define unique molecular characteristics of spinal ependymomas
title_sort expression alterations define unique molecular characteristics of spinal ependymomas
topic ependymoma
gene expression
meta-analysis
co-expression network
url https://eprints.nottingham.ac.uk/31769/
https://eprints.nottingham.ac.uk/31769/
https://eprints.nottingham.ac.uk/31769/