Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing

The recently released draft horse genome is incompletely characterised in terms of its repetitive element profile. This paper presents characterisation of the endogenous retrovirus (ERVs) of the horse genome based on a data-mining strategy using murine leukaemia virus proteins as queries. 978 ERV...

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Main Authors: Brown, Katherine, Moreton, Joanna, Malla, Sunir, Aboobaker, A. Aziz, Emes, Richard D., Tarlinton, Rachael E.
Format: Article
Published: Elsevier 2012
Online Access:https://eprints.nottingham.ac.uk/3169/
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author Brown, Katherine
Moreton, Joanna
Malla, Sunir
Aboobaker, A. Aziz
Emes, Richard D.
Tarlinton, Rachael E.
author_facet Brown, Katherine
Moreton, Joanna
Malla, Sunir
Aboobaker, A. Aziz
Emes, Richard D.
Tarlinton, Rachael E.
author_sort Brown, Katherine
building Nottingham Research Data Repository
collection Online Access
description The recently released draft horse genome is incompletely characterised in terms of its repetitive element profile. This paper presents characterisation of the endogenous retrovirus (ERVs) of the horse genome based on a data-mining strategy using murine leukaemia virus proteins as queries. 978 ERV gene sequences were identified. Sequences were identified from the gamma, epsilon and betaretrovirus genera. At least one full length gammaretroviral locus was identified, though the gammaretroviral sequences are very degenerate. Using these data the RNA expression of these ERVs were derived from RNA transcriptome data from a variety of equine tissues. Unlike the well studied human and murine ERVs there do not appear to be particular phylogenetic groups of equine ERVs that are more transcriptionally active. Using this novel approach provided a more technically feasible method to characterise ERV expression than previous studies.
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publishDate 2012
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spelling nottingham-31692020-05-04T20:22:18Z https://eprints.nottingham.ac.uk/3169/ Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing Brown, Katherine Moreton, Joanna Malla, Sunir Aboobaker, A. Aziz Emes, Richard D. Tarlinton, Rachael E. The recently released draft horse genome is incompletely characterised in terms of its repetitive element profile. This paper presents characterisation of the endogenous retrovirus (ERVs) of the horse genome based on a data-mining strategy using murine leukaemia virus proteins as queries. 978 ERV gene sequences were identified. Sequences were identified from the gamma, epsilon and betaretrovirus genera. At least one full length gammaretroviral locus was identified, though the gammaretroviral sequences are very degenerate. Using these data the RNA expression of these ERVs were derived from RNA transcriptome data from a variety of equine tissues. Unlike the well studied human and murine ERVs there do not appear to be particular phylogenetic groups of equine ERVs that are more transcriptionally active. Using this novel approach provided a more technically feasible method to characterise ERV expression than previous studies. Elsevier 2012 Article PeerReviewed Brown, Katherine, Moreton, Joanna, Malla, Sunir, Aboobaker, A. Aziz, Emes, Richard D. and Tarlinton, Rachael E. (2012) Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing. Virology, 433 (1). pp. 55-63. ISSN 0042-6822 http://www.sciencedirect.com/science/article/pii/S0042682212003595 doi:10.1016/j.virol.2012.07.010 doi:10.1016/j.virol.2012.07.010
spellingShingle Brown, Katherine
Moreton, Joanna
Malla, Sunir
Aboobaker, A. Aziz
Emes, Richard D.
Tarlinton, Rachael E.
Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title_full Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title_fullStr Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title_full_unstemmed Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title_short Characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
title_sort characterisation of retroviruses in the horse genome and their transcriptional activity via transcriptome sequencing
url https://eprints.nottingham.ac.uk/3169/
https://eprints.nottingham.ac.uk/3169/
https://eprints.nottingham.ac.uk/3169/