Response of Cupriavidus metallidurans CH34 to metals

Cupriavidus metallidurans CH34 displays resistance to a plethora of metals. Its response and underlying genetic determinants are dissected and detailed metal by metal (from arsenic to zinc). An important role for its megaplasmids pMOL28 and pMOL30 is shown, with high level resistance to cadmium, chr...

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Main Authors: Monsieurs, Pieter, Hobman, Jon L., Vandenbussche, Guy, Mergeay, Max, Van Hoult, Rob
Other Authors: Mergay, Max
Format: Book Section
Published: Springer 2015
Subjects:
Online Access:https://eprints.nottingham.ac.uk/31640/
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author Monsieurs, Pieter
Hobman, Jon L.
Vandenbussche, Guy
Mergeay, Max
Van Hoult, Rob
author2 Mergay, Max
author_facet Mergay, Max
Monsieurs, Pieter
Hobman, Jon L.
Vandenbussche, Guy
Mergeay, Max
Van Hoult, Rob
author_sort Monsieurs, Pieter
building Nottingham Research Data Repository
collection Online Access
description Cupriavidus metallidurans CH34 displays resistance to a plethora of metals. Its response and underlying genetic determinants are dissected and detailed metal by metal (from arsenic to zinc). An important role for its megaplasmids pMOL28 and pMOL30 is shown, with high level resistance to cadmium, chromate, cobalt, copper, mercury, nickel, lead and zinc mediated by well-known genes for detoxification often accompanied by other functions linked to acute or chronic stress. Nevertheless, metal resistance determinants are also found on the chromid (e.g. to chromate, copper and zinc) as well as on a large genomic island integrated in the chromosome (e.g. to cadmium, lead and mercury), even the core genome participates in certain responses such as to gold or selenium. Next, we summarized the environmental applications, which were developed based on the knowledge gained by studying these different determinants, and in particular biosensors and soil and water bioremediation. Finally, the general transcriptional response of C. metallidurans to sixteen different metals supplied at different concentrations (including acute stress) is discussed within the framework of its intricate regulatory network.
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spelling nottingham-316402020-05-04T20:11:33Z https://eprints.nottingham.ac.uk/31640/ Response of Cupriavidus metallidurans CH34 to metals Monsieurs, Pieter Hobman, Jon L. Vandenbussche, Guy Mergeay, Max Van Hoult, Rob Cupriavidus metallidurans CH34 displays resistance to a plethora of metals. Its response and underlying genetic determinants are dissected and detailed metal by metal (from arsenic to zinc). An important role for its megaplasmids pMOL28 and pMOL30 is shown, with high level resistance to cadmium, chromate, cobalt, copper, mercury, nickel, lead and zinc mediated by well-known genes for detoxification often accompanied by other functions linked to acute or chronic stress. Nevertheless, metal resistance determinants are also found on the chromid (e.g. to chromate, copper and zinc) as well as on a large genomic island integrated in the chromosome (e.g. to cadmium, lead and mercury), even the core genome participates in certain responses such as to gold or selenium. Next, we summarized the environmental applications, which were developed based on the knowledge gained by studying these different determinants, and in particular biosensors and soil and water bioremediation. Finally, the general transcriptional response of C. metallidurans to sixteen different metals supplied at different concentrations (including acute stress) is discussed within the framework of its intricate regulatory network. Springer Mergay, Max Van Houdt, Rob 2015 Book Section PeerReviewed Monsieurs, Pieter, Hobman, Jon L., Vandenbussche, Guy, Mergeay, Max and Van Hoult, Rob (2015) Response of Cupriavidus metallidurans CH34 to metals. In: Metal response in Cupriavidus metallidurans Volume 1: From habitats to genes and proteins. SpringerBriefs in biometals, 1 . Springer, Cham, pp. 45-89. ISBN 978331920593-9 Applied microbiology Microbiology Biogeosciences Environmental engineering Biotechnology http://www.springer.com/gb/book/9783319205939 doi:10.1007/978-3-319-20594-6 doi:10.1007/978-3-319-20594-6
spellingShingle Applied microbiology
Microbiology
Biogeosciences
Environmental engineering
Biotechnology
Monsieurs, Pieter
Hobman, Jon L.
Vandenbussche, Guy
Mergeay, Max
Van Hoult, Rob
Response of Cupriavidus metallidurans CH34 to metals
title Response of Cupriavidus metallidurans CH34 to metals
title_full Response of Cupriavidus metallidurans CH34 to metals
title_fullStr Response of Cupriavidus metallidurans CH34 to metals
title_full_unstemmed Response of Cupriavidus metallidurans CH34 to metals
title_short Response of Cupriavidus metallidurans CH34 to metals
title_sort response of cupriavidus metallidurans ch34 to metals
topic Applied microbiology
Microbiology
Biogeosciences
Environmental engineering
Biotechnology
url https://eprints.nottingham.ac.uk/31640/
https://eprints.nottingham.ac.uk/31640/
https://eprints.nottingham.ac.uk/31640/