Construction of a doxycycline inducible adipogenic lentiviral expression system
To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxyc...
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| Format: | Article |
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Elsevier
2013
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| Online Access: | https://eprints.nottingham.ac.uk/3158/ |
| _version_ | 1848790965467217920 |
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| author | Liu, Q. Hill, P.J. Karamitri, Angeliki Ryan, K.J.P. Chen, H.Y. Lomax, Michael A. |
| author_facet | Liu, Q. Hill, P.J. Karamitri, Angeliki Ryan, K.J.P. Chen, H.Y. Lomax, Michael A. |
| author_sort | Liu, Q. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes. |
| first_indexed | 2025-11-14T18:21:00Z |
| format | Article |
| id | nottingham-3158 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:21:00Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-31582020-05-04T20:19:42Z https://eprints.nottingham.ac.uk/3158/ Construction of a doxycycline inducible adipogenic lentiviral expression system Liu, Q. Hill, P.J. Karamitri, Angeliki Ryan, K.J.P. Chen, H.Y. Lomax, Michael A. To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes. Elsevier 2013-01 Article PeerReviewed Liu, Q., Hill, P.J., Karamitri, Angeliki, Ryan, K.J.P., Chen, H.Y. and Lomax, Michael A. (2013) Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid, 69 (1). pp. 96-103. ISSN 0147-619X Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte http://www.sciencedirect.com/science/article/pii/S0147619X12001060 doi:10.1016/j.plasmid.2012.10.001 doi:10.1016/j.plasmid.2012.10.001 |
| spellingShingle | Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte Liu, Q. Hill, P.J. Karamitri, Angeliki Ryan, K.J.P. Chen, H.Y. Lomax, Michael A. Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title | Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title_full | Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title_fullStr | Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title_full_unstemmed | Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title_short | Construction of a doxycycline inducible adipogenic lentiviral expression system |
| title_sort | construction of a doxycycline inducible adipogenic lentiviral expression system |
| topic | Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte |
| url | https://eprints.nottingham.ac.uk/3158/ https://eprints.nottingham.ac.uk/3158/ https://eprints.nottingham.ac.uk/3158/ |