Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms
Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing the reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are a major cause of meningococcal disease. High resolution genetic analyses of carriage and diseas...
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Oxford University Press
2016
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| Online Access: | https://eprints.nottingham.ac.uk/31313/ |
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| author | Oldfield, Neil J. Harrison, Odile B. Bayliss, Christopher D. Maiden, Martin C.J. Ala'Aldeen, Dlawer A.A. Turner, David P.J. |
| author_facet | Oldfield, Neil J. Harrison, Odile B. Bayliss, Christopher D. Maiden, Martin C.J. Ala'Aldeen, Dlawer A.A. Turner, David P.J. |
| author_sort | Oldfield, Neil J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing the reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are a major cause of meningococcal disease. High resolution genetic analyses of carriage and disease isolates can establish epidemiological relationships and identify potential virulence factors.
Methods. Whole genome sequence data were obtained from UK MenY carriage isolates from 1997-2010 (n=99). Sequences were compared to those from MenY invasive isolates from 2010 and 2011 (n=73) using a gene-by-gene approach.
Results. Comparisons across 1,605 core genes resolved 91% of isolates into one of eight clusters containing closely related disease and carriage isolates. Six clusters contained carried meningococci isolated in 1997-2001 suggesting temporal stability. One cluster of isolates, predominately sharing the designation Y: P1.5-1,10-1: F4-1: ST-1655 (cc23), was resolved into a sub-cluster with 86% carriage isolates and a second with 90% invasive isolates. These subclusters were defined by specific allelic differences in five core genes encoding glycerate kinase (glxK), valine-pyruvate transaminase (avtA), superoxide dismutase (sodB) and two hypothetical proteins.
Conclusions. High resolution genetic analyses detected long-term temporal stability and temporally-overlapping carriage and disease populations for MenY clones but also evidence of a disease-associated clone. |
| first_indexed | 2025-11-14T19:12:00Z |
| format | Article |
| id | nottingham-31313 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:12:00Z |
| publishDate | 2016 |
| publisher | Oxford University Press |
| recordtype | eprints |
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| spelling | nottingham-313132020-05-04T17:33:09Z https://eprints.nottingham.ac.uk/31313/ Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms Oldfield, Neil J. Harrison, Odile B. Bayliss, Christopher D. Maiden, Martin C.J. Ala'Aldeen, Dlawer A.A. Turner, David P.J. Background. Neisseria meningitidis is a frequent colonizer of the human nasopharynx with asymptomatic carriage providing the reservoir for invasive, disease-causing strains. Serogroup Y (MenY) strains are a major cause of meningococcal disease. High resolution genetic analyses of carriage and disease isolates can establish epidemiological relationships and identify potential virulence factors. Methods. Whole genome sequence data were obtained from UK MenY carriage isolates from 1997-2010 (n=99). Sequences were compared to those from MenY invasive isolates from 2010 and 2011 (n=73) using a gene-by-gene approach. Results. Comparisons across 1,605 core genes resolved 91% of isolates into one of eight clusters containing closely related disease and carriage isolates. Six clusters contained carried meningococci isolated in 1997-2001 suggesting temporal stability. One cluster of isolates, predominately sharing the designation Y: P1.5-1,10-1: F4-1: ST-1655 (cc23), was resolved into a sub-cluster with 86% carriage isolates and a second with 90% invasive isolates. These subclusters were defined by specific allelic differences in five core genes encoding glycerate kinase (glxK), valine-pyruvate transaminase (avtA), superoxide dismutase (sodB) and two hypothetical proteins. Conclusions. High resolution genetic analyses detected long-term temporal stability and temporally-overlapping carriage and disease populations for MenY clones but also evidence of a disease-associated clone. Oxford University Press 2016-01-07 Article PeerReviewed Oldfield, Neil J., Harrison, Odile B., Bayliss, Christopher D., Maiden, Martin C.J., Ala'Aldeen, Dlawer A.A. and Turner, David P.J. (2016) Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms. Journal of Infectious Diseases . pp. 1-9. ISSN 1537-6613 Neisseria Meningitidis Whole Genome Sequencing Carriage Serogroup Y Epidemiology http://jid.oxfordjournals.org/content/early/2016/02/03/infdis.jiw008 doi:10.1093/infdis/jiw008 doi:10.1093/infdis/jiw008 |
| spellingShingle | Neisseria Meningitidis Whole Genome Sequencing Carriage Serogroup Y Epidemiology Oldfield, Neil J. Harrison, Odile B. Bayliss, Christopher D. Maiden, Martin C.J. Ala'Aldeen, Dlawer A.A. Turner, David P.J. Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title | Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title_full | Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title_fullStr | Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title_full_unstemmed | Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title_short | Genomic analysis of serogroup Y Neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| title_sort | genomic analysis of serogroup y neisseria meningitidis isolates reveals extensive similarities between carriage and disease-associated organisms |
| topic | Neisseria Meningitidis Whole Genome Sequencing Carriage Serogroup Y Epidemiology |
| url | https://eprints.nottingham.ac.uk/31313/ https://eprints.nottingham.ac.uk/31313/ https://eprints.nottingham.ac.uk/31313/ |