Different genome stability proteins underpin primed
and naïve adaptation in E. coli CRISPR-Cas immunity

QR Code

Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity

CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci, termed ‘Adaptation’, which is dependent on Cas1 and Cas2 proteins. In Escherichia coli, Cascade-Cas3 degrades invader DNA to effec...

Full description

Bibliographic Details
Main Authors:	Ivančić-Baće, Ivana, Cass, Simon, Wearne, Stephen J., Bolt, Edward L.
Format:	Article
Published:	Oxford University Press 2015
Online Access:	https://eprints.nottingham.ac.uk/31310/

Similar Items

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS
by: Majsec, Kristina, et al.
Published: (2016)

CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases
by: Killelea, Tom, et al.
Published: (2018)

CRISPR-Cas immunity: analysis of adaptation and interference reactions in prokaryotes
by: Cass, S.D.B.
Published: (2016)

The role of Cas8 in type I CRISPR interference
by: Cass, Simon, et al.
Published: (2015)

Analysis of Cas1-2 and Cas3 CRISPR immunity proteins in E. coli cell physiology
by: Reeves, Ryan Peter
Published: (2019)

Analysis of DNA binding and translocation by the E. coli CRISPR immunity protein Cas3
by: He, Liu
Published: (2018)

Characterising Host Factors Required for CRISPR Cas Adaptation in Escherichia coli
by: Edwards, Harry
Published: (2023)

Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System
by: Arooj, Mahira
Published: (2018)

CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing
by: Moradpour, Mahdi, et al.
Published: (2019)

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3
by: Gong, Bei, et al.
Published: (2014)

CRISPR-Cas9-mediated genome editing of the extracellular matrix gene TNC in human fibroblasts
by: Brunetti, Giulio
Published: (2017)

Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement
by: Shaharuddin, Noor Azmi, et al.
Published: (2024)

The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells
by: Yu, Choo Yee, et al.
Published: (2024)

Investigation of CRISPR-Cas adaptation mechanism through exploration of Cas4-1 fusions
by: Walker, Emily
Published: (2019)

Remodeling and control of homologous recombination by DNA helicases and translocases that target recombinases and synapsis
by: Northall, Sarah J, et al.
Published: (2016)

The expansion of available bioparts and the development of CRISPR/Cas9 for genetic engineering of Parageobacillus thermoglucosidasius
by: Lau, Matthew Simon Harold
Published: (2019)

Analysis of the E. coli CRISPR Cas3 protein and its potential for regulation by a short non-coding RNA
by: Kennerdale, Niamh
Published: (2024)

Harnessing CRISPR-Cas to combat COVID-19: from diagnostics to therapeutics
by: Kok, Gan Chan, et al.
Published: (2021)

CRISPR-Cas knockout of miR21 reduces glioma growth
by: Nieland, Lisa, et al.
Published: (2022)

The enhancement of plant disease resistance using CRISPR/Cas9 technology
by: Mohd Azman, Amier Asyraf, et al.
Published: (2022)

DNA-free CRISPR/Cas9 genome editing system for oil palm protoplasts using multiple ribonucleoproteins (RNPs) complexes
by: Norfaezah, Jamaludin, et al.
Published: (2024)

Targeting BCL11A/nuclear receptor complexes in breast tumour cells using CRISPR-Cas9 genome editing tool
by: Clemente Olivo, Maria del Pilar
Published: (2017)

Disease modeling hypertrophic cardiomyopathy using CRISPR/Cas9 genome editing technology in human pluripotent stem cell-derived cardiomyocytes
by: Mosqueira, Diogo
Published: (2018)

CRISPR systems - molecular mechanisms of the interaction between Cas1 and DnaK
by: Stewart, Alison L.
Published: (2025)

Applications of a high-throughput screen detecting CRISPR-Cas spacer acquisition
by: Cannon, Christopher
Published: (2025)

Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
by: Ravichantar, Nithya
Published: (2020)

Targeting androgen receptor and PIP5K1A in prostate cancer cells using CRISPR-Cas9 genome editing tool
by: Hernández-Quiles, Miguel
Published: (2017)

Development of CRISPR-cas9 ribonucleoprotein complex platform for disruption of candidalysin in Candida albicans
by: Ahmad, Hasna, et al.
Published: (2020)

Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
by: Eftekhari, Fatima, et al.
Published: (2018)

Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein
by: Hooton, Steven P.T., et al.
Published: (2015)

A refined method to study gene dosage changes in-vitro using CRISPR/Cas9
by: Raposo, T.P., et al.
Published: (2017)

Part A: Application of fluorescent markers onto CRISPR-associated proteins/ Part B: Substitution of cysteine residues in CRISPR associated Protein Cas1
by: Parr, Lochlan
Published: (2024)

Development of CRISPR-Cas9-mediated site-directed mutagenesis of ECE1 gene (candidalysin) in Candida albicans
by: Engku Nasrullah Satiman, Engku Anis Fariha, et al.
Published: (2020)

Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system
by: Garcia-Bloj, B., et al.
Published: (2016)

Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
by: Kondrashov, Alexander, et al.
Published: (2018)

Harnessing CRISPR/Cas9 system to engineer disease resistance in solanaceous crops: current progress and future prospects
by: Ahmed, Shiuli, et al.
Published: (2025)

Immunization in Alzheimer’s disease: Naïve hope or realistic clinical potential?
by: Foster, Jonathan, et al.
Published: (2009)

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs
by: Bertero, Alessandro, et al.
Published: (2016)

Correction Of Glucose-6-Phosphate Dehydrogenase (G6PD) Viangchan Mutation In Monocytes Using CRISPR/CAS9
by: Vengidasan, Lelamekala
Published: (2018)

Functional interrogation of CRISPR-CAS9 mediated EpCAM knockout in breast cancer cells / Afiqah Fasihah Abdul Rahim
by: Afiqah Fasihah , Abdul Rahim
Published: (2022)