Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry
A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microsc...
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| Format: | Article |
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Wiley-VCH Verlag
2015
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| Online Access: | https://eprints.nottingham.ac.uk/31291/ |
| _version_ | 1848794169212928000 |
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| author | Herod, Morgan R. Pineda, Robert G. Mautner, Vivien Onion, David |
| author_facet | Herod, Morgan R. Pineda, Robert G. Mautner, Vivien Onion, David |
| author_sort | Herod, Morgan R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors. |
| first_indexed | 2025-11-14T19:11:55Z |
| format | Article |
| id | nottingham-31291 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:11:55Z |
| publishDate | 2015 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-312912020-05-04T17:02:30Z https://eprints.nottingham.ac.uk/31291/ Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry Herod, Morgan R. Pineda, Robert G. Mautner, Vivien Onion, David A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors. Wiley-VCH Verlag 2015-02-18 Article PeerReviewed Herod, Morgan R., Pineda, Robert G., Mautner, Vivien and Onion, David (2015) Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry. Small, 11 (7). pp. 797-803. ISSN 1613-6829 quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry http://onlinelibrary.wiley.com/doi/10.1002/smll.201401885/full doi:10.1002/smll.201401885 doi:10.1002/smll.201401885 |
| spellingShingle | quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry Herod, Morgan R. Pineda, Robert G. Mautner, Vivien Onion, David Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title | Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title_full | Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title_fullStr | Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title_full_unstemmed | Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title_short | Quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| title_sort | quantum dot labelling of adenovirus allows for highly sensitive single cell flow and imaging cytometry |
| topic | quantum dots; adenovirus; gene therapy; flow cytometry; imaging cytometry |
| url | https://eprints.nottingham.ac.uk/31291/ https://eprints.nottingham.ac.uk/31291/ https://eprints.nottingham.ac.uk/31291/ |