Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films

Probiotic incorporation in edible films and coatings has been shown recently to be an efficient strategy for the delivery of probiotics in foods. In the present work, the impact of the compositional, physicochemical and structural properties of binary starch-protein edible films on Lactobacillus rha...

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Main Authors: Soukoulis, Christos, Singh, Poonam, Macnaughtan, William, Parmenter, Christopher D.J., Fisk, Ian D.
Format: Article
Published: Elsevier 2015
Subjects:
Online Access:https://eprints.nottingham.ac.uk/31229/
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author Soukoulis, Christos
Singh, Poonam
Macnaughtan, William
Parmenter, Christopher D.J.
Fisk, Ian D.
author_facet Soukoulis, Christos
Singh, Poonam
Macnaughtan, William
Parmenter, Christopher D.J.
Fisk, Ian D.
author_sort Soukoulis, Christos
building Nottingham Research Data Repository
collection Online Access
description Probiotic incorporation in edible films and coatings has been shown recently to be an efficient strategy for the delivery of probiotics in foods. In the present work, the impact of the compositional, physicochemical and structural properties of binary starch-protein edible films on Lactobacillus rhamnosus GG viability and stability was evaluated. Native rice and corn starch, as well as bovine skin gelatine, sodium caseinate and soy protein concentrate were used for the fabrication of the probiotic edible films. Starch and protein type both impacted the structural, mechanical, optical and thermal properties of the films, and the process loss of L. rhamnosus GG during evaporation-dehydration was significantly lower in the presence of proteins (0.91–1.07 log CFU/g) compared to solely starch based systems (1.71 log CFU/g). A synergistic action between rice starch and proteins was detected when monitoring the viability of L. rhamnosus GG over four weeks at fridge and room temperature conditions. In particular, a 3- to 7-fold increase in the viability of L. rhamnosus GG was observed in the presence of proteins, with sodium caseinate – rice starch based films offering the most enhanced stability. The film's shelf-life (as calculated using the FAO/WHO (2011) basis of 6 log viable CFU/g) ranged between 27-96 and 15–24 days for systems stored at fridge or room temperature conditions respectively.
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spelling nottingham-312292020-05-04T17:14:26Z https://eprints.nottingham.ac.uk/31229/ Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films Soukoulis, Christos Singh, Poonam Macnaughtan, William Parmenter, Christopher D.J. Fisk, Ian D. Probiotic incorporation in edible films and coatings has been shown recently to be an efficient strategy for the delivery of probiotics in foods. In the present work, the impact of the compositional, physicochemical and structural properties of binary starch-protein edible films on Lactobacillus rhamnosus GG viability and stability was evaluated. Native rice and corn starch, as well as bovine skin gelatine, sodium caseinate and soy protein concentrate were used for the fabrication of the probiotic edible films. Starch and protein type both impacted the structural, mechanical, optical and thermal properties of the films, and the process loss of L. rhamnosus GG during evaporation-dehydration was significantly lower in the presence of proteins (0.91–1.07 log CFU/g) compared to solely starch based systems (1.71 log CFU/g). A synergistic action between rice starch and proteins was detected when monitoring the viability of L. rhamnosus GG over four weeks at fridge and room temperature conditions. In particular, a 3- to 7-fold increase in the viability of L. rhamnosus GG was observed in the presence of proteins, with sodium caseinate – rice starch based films offering the most enhanced stability. The film's shelf-life (as calculated using the FAO/WHO (2011) basis of 6 log viable CFU/g) ranged between 27-96 and 15–24 days for systems stored at fridge or room temperature conditions respectively. Elsevier 2015-08-30 Article PeerReviewed Soukoulis, Christos, Singh, Poonam, Macnaughtan, William, Parmenter, Christopher D.J. and Fisk, Ian D. (2015) Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films. Food Hydrocolloids, 52 . pp. 876-887. ISSN 1873-7137 Probiotics Rice Starch Corn Starch Gelatine Sodium Caseinate Soy Protein http://www.sciencedirect.com/science/article/pii/S0268005X15300709 doi:10.1016/j.foodhyd.2015.08.025 doi:10.1016/j.foodhyd.2015.08.025
spellingShingle Probiotics
Rice Starch
Corn Starch
Gelatine
Sodium Caseinate
Soy Protein
Soukoulis, Christos
Singh, Poonam
Macnaughtan, William
Parmenter, Christopher D.J.
Fisk, Ian D.
Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title_full Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title_fullStr Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title_full_unstemmed Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title_short Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films
title_sort compositional and physicochemical factors governing the viability of lactobacillus rhamnosus gg embedded in starch-protein based edible films
topic Probiotics
Rice Starch
Corn Starch
Gelatine
Sodium Caseinate
Soy Protein
url https://eprints.nottingham.ac.uk/31229/
https://eprints.nottingham.ac.uk/31229/
https://eprints.nottingham.ac.uk/31229/