Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes
AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wid...
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| Format: | Article |
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Future Medicine
2016
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| Online Access: | https://eprints.nottingham.ac.uk/30944/ |
| _version_ | 1848794096147103744 |
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| author | Glossop, John R. Emes, Richard D. Nixon, Nicola B. Packham, Jon C. Fryer, Anthony A. Mattey, Derek L. Farrell, William E. |
| author_facet | Glossop, John R. Emes, Richard D. Nixon, Nicola B. Packham, Jon C. Fryer, Anthony A. Mattey, Derek L. Farrell, William E. |
| author_sort | Glossop, John R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA.
PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals.
RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease.
CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA. |
| first_indexed | 2025-11-14T19:10:45Z |
| format | Article |
| id | nottingham-30944 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:10:45Z |
| publishDate | 2016 |
| publisher | Future Medicine |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-309442020-05-04T17:28:40Z https://eprints.nottingham.ac.uk/30944/ Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes Glossop, John R. Emes, Richard D. Nixon, Nicola B. Packham, Jon C. Fryer, Anthony A. Mattey, Derek L. Farrell, William E. AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA. PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals. RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease. CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA. Future Medicine 2016-02-01 Article PeerReviewed Glossop, John R., Emes, Richard D., Nixon, Nicola B., Packham, Jon C., Fryer, Anthony A., Mattey, Derek L. and Farrell, William E. (2016) Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes. Epigenomics, 8 (2). pp. 209-224. ISSN 1750-192X Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array. http://www.futuremedicine.com/doi/10.2217/epi.15.103 doi:10.2217/epi.15.103 doi:10.2217/epi.15.103 |
| spellingShingle | Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array. Glossop, John R. Emes, Richard D. Nixon, Nicola B. Packham, Jon C. Fryer, Anthony A. Mattey, Derek L. Farrell, William E. Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title | Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title_full | Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title_fullStr | Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title_full_unstemmed | Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title_short | Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes |
| title_sort | genome-wide profiling in treatment-naive early rheumatoid arthritis reveals dna methylome changes in t and b lymphocytes |
| topic | Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array. |
| url | https://eprints.nottingham.ac.uk/30944/ https://eprints.nottingham.ac.uk/30944/ https://eprints.nottingham.ac.uk/30944/ |