Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis are powerful ways to study mobility and stoichiometry of G protein coupled receptor complexes, within microdomains of single living cells. However, relating these properties to molecular mechanisms can be challe...
| Main Authors: | Kilpatrick, Laura E., Briddon, Stephen J., Holliday, Nicholas D. |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2012
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/3067/ |
Similar Items
Identification of a Cyanine-dye labeled peptidic ligand for Y₁R and Y₄R, based upon the Neuropeptide Y C-terminal analogue, BVD-15
by: Liu, Mengjie, et al.
Published: (2016)
by: Liu, Mengjie, et al.
Published: (2016)
Direct visualisation of internalization of the adenosine A3 receptor and localization with arrestin3 using a fluorescent agonist
by: Stoddart, Leigh A., et al.
Published: (2015)
by: Stoddart, Leigh A., et al.
Published: (2015)
The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors
by: Kilpatrick, Laura E., et al.
Published: (2016)
by: Kilpatrick, Laura E., et al.
Published: (2016)
The use of bimolecular fluorescence complementation (BiFC) to investigate the functional implications of neuropeptide Y receptor dimerisation and beta-arrestin recruitment
by: Kilpatrick, Laura Elise
Published: (2015)
by: Kilpatrick, Laura Elise
Published: (2015)
Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925
by: Conroy, Sean, et al.
Published: (2018)
by: Conroy, Sean, et al.
Published: (2018)
Kinetic analysis of antagonist-occupied adenosine-A3 receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism
by: Corriden, Ross, et al.
Published: (2014)
by: Corriden, Ross, et al.
Published: (2014)
Design and evaluation of fluorescent and non-fluorescent bivalent ligands to explore their mode of action at neuropeptide Y receptors
by: Richardson, Rachel
Published: (2019)
by: Richardson, Rachel
Published: (2019)
Fluorescence quenching of optical fiber chemical sensor detecting hazardous dinitrobenzene material
by: Yusufu, Abdullahi Magaji
Published: (2017)
by: Yusufu, Abdullahi Magaji
Published: (2017)
Design, Synthesis, and Evaluation of New P2Y2 Receptor Antagonists and Fluorescent Ligands
by: Knight, Rebecca
Published: (2025)
by: Knight, Rebecca
Published: (2025)
Optimisation of Peptide Linker-based Fluorescent Ligands for the Histamine H1 Receptor
by: Kok, Zhi Yuan
Published: (2020)
by: Kok, Zhi Yuan
Published: (2020)
An appraisal of the Suzuki cross-coupling reaction for the synthesis of novel fluorescent coumarin derivatives
by: Alhaj Zen, Amer, et al.
Published: (2014)
by: Alhaj Zen, Amer, et al.
Published: (2014)
Small molecule-based fluorescent probes as tool compounds for chemokine receptors CXCR4 and ACKR3
by: Dekkers, Sebastian
Published: (2019)
by: Dekkers, Sebastian
Published: (2019)
Development of novel fluorescent dye molecules based on BODIPY
by: Hassanain, Hawazen
Published: (2019)
by: Hassanain, Hawazen
Published: (2019)
Dimerization of ABCG2 analysed by bimolecular fluorescence complementation
by: Haider, Ameena J., et al.
Published: (2011)
by: Haider, Ameena J., et al.
Published: (2011)
Dielectric and fluorescence spectroscopy techniques for determination of palm olein degradation during batch deep fat frying
by: Ibrahim, Nur Ul Atikah
Published: (2017)
by: Ibrahim, Nur Ul Atikah
Published: (2017)
Novel complementation biosensors to investigate G protein-coupled receptor kinetics and signalling bias
by: Dijon, Nicola C
Published: (2022)
by: Dijon, Nicola C
Published: (2022)
Augmenting automated analytics using fluorescent nanosensors
by: Chauhan, Veeren M
Published: (2018)
by: Chauhan, Veeren M
Published: (2018)
Detection of lard via fluorescent fiber probe sensor utilizing graphene quantum dots
by: Che Lah, Che Nur Hamizah
Published: (2018)
by: Che Lah, Che Nur Hamizah
Published: (2018)
Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques
by: Wong, Kelvin, et al.
Published: (2016)
by: Wong, Kelvin, et al.
Published: (2016)
Design, synthesis and pharmacological evaluation of novel fluorescent ligands for the mu-opioid receptor
by: Marshall, William
Published: (2021)
by: Marshall, William
Published: (2021)
Illuminating the life of the adenosine receptors: development and application of subtype-selective fluorescent probes for the study of the human A1 and A2A adenosine receptors in living cells
by: Comeo, Eleonora
Published: (2022)
by: Comeo, Eleonora
Published: (2022)
Non-destructive estimation of oil palm yield and quality with fluorescence sensing
by: Abu Kassim, Norul Husna
Published: (2016)
by: Abu Kassim, Norul Husna
Published: (2016)
Investigating the use of novel fluorescent nanosensors to measure chemical changes in bacterial surroundings
by: Perkins, Mark Edwin
Published: (2021)
by: Perkins, Mark Edwin
Published: (2021)
Production and electrophoresis-based purification of recombinant green fluorescent protein from Escherichia coli
by: Chew, Few Ne
Published: (2011)
by: Chew, Few Ne
Published: (2011)
Neuropeptides, amines and amine receptors in the human spinal cord: the effects of Parkinson's disease
by: Cooper, Cindy L.
Published: (1989)
by: Cooper, Cindy L.
Published: (1989)
Far-field resonance fluorescence from a dipole-interacting laser-driven cold atomic gas
by: Jones, Ryan, et al.
Published: (2016)
by: Jones, Ryan, et al.
Published: (2016)
The growth and fluorescence of organic monolayers and heterostructures
by: Kerfoot, James
Published: (2018)
by: Kerfoot, James
Published: (2018)
Modulation of mu-opioid receptor dynamics at the plasma membrane and its implications for signalling and trafficking
by: Underwood, Owen George
Published: (2024)
by: Underwood, Owen George
Published: (2024)
Improved resolution and signal-to-noise ratio performance of a confocal fluorescence microscope
by: Kakade, Rohan
Published: (2016)
by: Kakade, Rohan
Published: (2016)
Fluorescence and ZEKE spectroscopy of small, jet-cooled aromatic molecules
by: Davies, Alexander R.
Published: (2022)
by: Davies, Alexander R.
Published: (2022)
The design, synthesis and characterisation of subtype selective dipeptide-linked fluorescent ligands for human β1 and β2–adrenoceptors
by: Santu, L.T.
Published: (2017)
by: Santu, L.T.
Published: (2017)
Investigation into the effect of formulation on intravenous lipid emulsion metabolism using a novel in vitro fluorescent assay
by: Dougall, Paul W.R.
Published: (2016)
by: Dougall, Paul W.R.
Published: (2016)
Development of a time gated, compressive detection Raman instrument for effective fluorescence rejection and multilayer analysis
by: Corden, Christopher James
Published: (2021)
by: Corden, Christopher James
Published: (2021)
Energy saving controller for fluorescent lamps
by: Cheong, Zhi Xiong
Published: (2010)
by: Cheong, Zhi Xiong
Published: (2010)
Optimising performance of a confocal fluorescence microscope with a differential pinhole
by: Kakade, Rohan, et al.
Published: (2015)
by: Kakade, Rohan, et al.
Published: (2015)
Analysis of ABCG2 pharmacology and oligomerisation by solution based fluorescence correlation spectroscopy
by: Horsey, Aaron J.
Published: (2019)
by: Horsey, Aaron J.
Published: (2019)
Rate of X chromosome aneuploidy in young fertile women: Comparison of cultured and uncultured cell preparations using fluorescence in situ hybridisation
by: Patton, K., et al.
Published: (2010)
by: Patton, K., et al.
Published: (2010)
Optimisation of transient green fluorescent protein (GFP) gene expression in Phalaenopsis Violacea Orchid mediated by Agrobacterium Tumefaciens-mediated transformation system
by: Julkifle, Advina L., et al.
Published: (2010)
by: Julkifle, Advina L., et al.
Published: (2010)
Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter
by: Yong, Wilson Thau Lim, et al.
Published: (2006)
by: Yong, Wilson Thau Lim, et al.
Published: (2006)
A proof-of-concept study into turmeric spice as an improvised fluorescent latent fingermark detection powder for limited resource jurisdictions
by: Lópes, T., et al.
Published: (2025)
by: Lópes, T., et al.
Published: (2025)
Similar Items
-
Identification of a Cyanine-dye labeled peptidic ligand for Y₁R and Y₄R, based upon the Neuropeptide Y C-terminal analogue, BVD-15
by: Liu, Mengjie, et al.
Published: (2016) -
Direct visualisation of internalization of the adenosine A3 receptor and localization with arrestin3 using a fluorescent agonist
by: Stoddart, Leigh A., et al.
Published: (2015) -
The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors
by: Kilpatrick, Laura E., et al.
Published: (2016) -
The use of bimolecular fluorescence complementation (BiFC) to investigate the functional implications of neuropeptide Y receptor dimerisation and beta-arrestin recruitment
by: Kilpatrick, Laura Elise
Published: (2015) -
Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925
by: Conroy, Sean, et al.
Published: (2018)