Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor
Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is bas...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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American Chemical Society
2015
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| Online Access: | https://eprints.nottingham.ac.uk/30647/ |
| _version_ | 1848794030011318272 |
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| author | Rawson, Frankie J. Hicks, Jacqueline Dodd, Nicholas Abate, Wondwossen Garrett, David J. Yip, Nga-Chi Fejer, Gyorgy Downard, Alison J. Baronian, Kim H.R. Jackson, Simon K. Mendes, Paula M. |
| author_facet | Rawson, Frankie J. Hicks, Jacqueline Dodd, Nicholas Abate, Wondwossen Garrett, David J. Yip, Nga-Chi Fejer, Gyorgy Downard, Alison J. Baronian, Kim H.R. Jackson, Simon K. Mendes, Paula M. |
| author_sort | Rawson, Frankie J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular “pulse” of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection. |
| first_indexed | 2025-11-14T19:09:42Z |
| format | Article |
| id | nottingham-30647 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:09:42Z |
| publishDate | 2015 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-306472020-05-04T17:18:37Z https://eprints.nottingham.ac.uk/30647/ Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor Rawson, Frankie J. Hicks, Jacqueline Dodd, Nicholas Abate, Wondwossen Garrett, David J. Yip, Nga-Chi Fejer, Gyorgy Downard, Alison J. Baronian, Kim H.R. Jackson, Simon K. Mendes, Paula M. Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular “pulse” of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection. American Chemical Society 2015-10-28 Article PeerReviewed Rawson, Frankie J., Hicks, Jacqueline, Dodd, Nicholas, Abate, Wondwossen, Garrett, David J., Yip, Nga-Chi, Fejer, Gyorgy, Downard, Alison J., Baronian, Kim H.R., Jackson, Simon K. and Mendes, Paula M. (2015) Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor. ACS Applied Materials and Interfaces, 7 (42). pp. 23527-23537. ISSN 1944-8252 Intracellular Sensor Electrocatalytic Sensor Carbon Nanotubes Reactive Oxygen Species Hydrogen Peroxide Macrophage Cells http://pubs.acs.org/doi/abs/10.1021/acsami.5b06493 doi:10.1021/acsami.5b06493 doi:10.1021/acsami.5b06493 |
| spellingShingle | Intracellular Sensor Electrocatalytic Sensor Carbon Nanotubes Reactive Oxygen Species Hydrogen Peroxide Macrophage Cells Rawson, Frankie J. Hicks, Jacqueline Dodd, Nicholas Abate, Wondwossen Garrett, David J. Yip, Nga-Chi Fejer, Gyorgy Downard, Alison J. Baronian, Kim H.R. Jackson, Simon K. Mendes, Paula M. Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title | Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title_full | Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title_fullStr | Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title_full_unstemmed | Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title_short | Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| title_sort | fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor |
| topic | Intracellular Sensor Electrocatalytic Sensor Carbon Nanotubes Reactive Oxygen Species Hydrogen Peroxide Macrophage Cells |
| url | https://eprints.nottingham.ac.uk/30647/ https://eprints.nottingham.ac.uk/30647/ https://eprints.nottingham.ac.uk/30647/ |