Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets

QR Code

Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets

Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligands. This process, commonly referred to as next gene...

Full description

Bibliographic Details
Main Authors:	Spiliotopoulos, Anastasios, Owen, Jonathan P., Maddison, Ben C., Dreveny, Ingrid, Rees, Helen C., Gough, Kevin C.
Format:	Article
Published:	Elsevier 2015
Subjects:	Phage display; ScFv; Rescue; Inverse PCR; CDR3; DNA sequencing
Online Access:	https://eprints.nottingham.ac.uk/30247/

Similar Items

Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells
by: Gough, Kevin C., et al.
Published: (2015)

Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris
by: de la Cruz, Silvia, et al.
Published: (2016)

Isolating novel antibodies against Eimeria infections of chickens using Next Generation Phage Display and conventional Phage Display
by: Angani, Mary Talatu
Published: (2020)

Isolating novel antibodies against difficult targets using next generation antibody phage display
by: Reader, Rose H
Published: (2019)

New approaches to study canine and equine influenza A virus
by: Leverett, H.E.
Published: (2025)

The development candidate therapeutic and diagnostic ligands for prion diseases
by: Workman, R.W.
Published: (2018)

Identification of host-pathogen interacting molecules of Campylobacter jejuni using phage display technology and in silico sequence analysis
by: Abruquah, Harry H.
Published: (2009)

Applications of phage peptide libraries for epitope discovery and identification of novel anti-virals
by: Hakami, Abdulrahim R.
Published: (2017)

Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk
by: Gerrard, Zara E., et al.
Published: (2018)

Virus deep sequencing: understanding the unknown
by: Rozado Aguirre, Zurine
Published: (2018)

The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology
by: Rees, Helen C., et al.
Published: (2014)

The discovery and characterisation of novel antimicrobial peptides against bacterial pathogens with porcine host specificity.
by: Clarke, Britany
Published: (2023)

Poor evacuation and flood safety measure for local victims in Pagoh Muar, Johor: implementing FRC package to aid / Nur Nabila Ab Sukor
by: Ab Sukor, Nur Nabila Ab Sukor
Published: (2014)

The unbanked and poverty: predicting area-level socio-economic vulnerability from M-Money transactions
by: Goulding, James, et al.
Published: (2018)

Exploring the diversity and ecology of bacteriophages in the dairy farm environment
by: Cook, Ryan
Published: (2023)

Expression, Characterization, and Evaluation of a RANK-binding Single Chain Fraction Variable: An Osteoclast Targeting Drug Delivery Strategy
by: Newa, M., et al.
Published: (2014)

Response Time Analysis of Fire and Rescue Services in Collaboration with Nottinghamshire Fire & Rescue Service
by: Tan, Melis
Published: (2019)

Investigation of antibody-based immune recognition of infections with Salmonella enterica serovars Typhimurium and Enteritidis
by: Naqid, Ibrahim
Published: (2016)

The application of eDNA for monitoring of the great crested newt in the UK
by: Rees, Helen C., et al.
Published: (2014)

Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
by: Rozado-Aguirre, Zuriñe, et al.
Published: (2016)

Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria
by: Swift, Benjamin, et al.
Published: (2014)

Quantitative Real-Time PCR in aDNA Research
by: Bunce, Michael, et al.
Published: (2012)

Opinions of UK rescue shelter and rehoming center workers on the problems facing their industry
by: Stavisky, Jenny, et al.
Published: (2017)

Application of a bacteriophage cocktail to reduce Salmonella Typhimurium U288 contamination on pig skin
by: Hooton, Steven P.T., et al.
Published: (2011)

Stochastic resonance in an RF SQUID with shunted ScS junction
by: Turutanov, O., et al.
Published: (2013)

Development of diagnostic assays for emergent RNA viruses
by: Wu, Ge
Published: (2020)

Molecular methods for the verification of bacterial decontamination
by: Willoughby, Sarah L.
Published: (2021)

Isolation and evolutionary analysis of Australasian topotype of bluetongue virus serotype 4 from India
by: Reddy, Y. Vishnuvardhan, et al.
Published: (2017)

Rescue PCI in the management of STEMI: Contemporary results from the Melbourne Interventional Group registry
by: Fernando, H., et al.
Published: (2021)

Classification of single cell types during leukemia therapy using artificial neural networks
by: LYU, Minjie, et al.
Published: (2021)

Discovery and characterisation of anti-amyloid beta fibril antibodies from naive phage display VHH libraries
by: Fernandez Bonfante, Juan
Published: (2022)

Investigating radiation responsiveness in rectal cancer
by: Hassall, James
Published: (2021)

Identification of stable reference genes for quantitative PCR in koalas
by: Sarker, N., et al.
Published: (2018)

Rescuing companies or their creditors: Understanding the role of the CVA as a rescue procedure in the UK
by: Morgan, Susan
Published: (2022)

Hierarchical Bayesian level set inversion
by: Dunlop, Matthew M., et al.
Published: (2016)

Acoustic response of reservoir sandstones during injection of supercritical CO2
by: Lebedev, Maxim, et al.
Published: (2014)

Biocontrol of V. cholerae using bacteriophage
by: Bhandare, Sudhakar Ganapati
Published: (2015)

Look Here! Measuring the Attentional Demand of Near-Future Full Windshield Vehicle Displays
by: Topliss, Bethan
Published: (2023)

Seismic processing, inversion, and AVO for gold exploration: Case study from Western Australia
by: Harrison, C., et al.
Published: (2012)

Clinical Evaluation of a MassCode PCR Assay for the Syndromic Detection of Pathogens that cause Respiratory Disease
by: Smith, D., et al.
Published: (2011)