MultiSig: a new high-precision approach to the analysis of complex biomolecular systems
MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing method...
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| Format: | Article |
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Springer Verlag
2013
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| Online Access: | https://eprints.nottingham.ac.uk/3003/ |
| _version_ | 1848790928892887040 |
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| author | Gillis, Richard B. Adams, Gary G. Heinze, Thomas Nikolajski, Melanie Harding, Stephen E. Rowe, Arthur J. |
| author_facet | Gillis, Richard B. Adams, Gary G. Heinze, Thomas Nikolajski, Melanie Harding, Stephen E. Rowe, Arthur J. |
| author_sort | Gillis, Richard B. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing methods of analysis through reducing the ‘noise’ level of certain important parameter estimates by up to orders of magnitude. Long-known limitations of the SE method, arising from lack of knowledge of the true fringe number in fringe optics and from the use of unstable numerical algorithms such as numerical differentiation, have been transcended. An approach to data analysis, akin to ‘spatial filtering’, has been developed, and shown by both simulation and practical application to be a powerful aid to the precision with which near-monodisperse systems can be analysed, potentially yielding information on protein-solvent interaction. For oligo- and poly-disperse systems the information returned includes precise average mass distributions over both cell radial and concentration ranges and mass-frequency histograms at fixed radial positions. The application of MultiSig analysis to various complex heterogenous systems and potentially multiply-interacting carbohydrate oligomers is described. |
| first_indexed | 2025-11-14T18:20:25Z |
| format | Article |
| id | nottingham-3003 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:20:25Z |
| publishDate | 2013 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-30032020-05-04T20:18:39Z https://eprints.nottingham.ac.uk/3003/ MultiSig: a new high-precision approach to the analysis of complex biomolecular systems Gillis, Richard B. Adams, Gary G. Heinze, Thomas Nikolajski, Melanie Harding, Stephen E. Rowe, Arthur J. MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe) system employed, thus supplanting existing methods of analysis through reducing the ‘noise’ level of certain important parameter estimates by up to orders of magnitude. Long-known limitations of the SE method, arising from lack of knowledge of the true fringe number in fringe optics and from the use of unstable numerical algorithms such as numerical differentiation, have been transcended. An approach to data analysis, akin to ‘spatial filtering’, has been developed, and shown by both simulation and practical application to be a powerful aid to the precision with which near-monodisperse systems can be analysed, potentially yielding information on protein-solvent interaction. For oligo- and poly-disperse systems the information returned includes precise average mass distributions over both cell radial and concentration ranges and mass-frequency histograms at fixed radial positions. The application of MultiSig analysis to various complex heterogenous systems and potentially multiply-interacting carbohydrate oligomers is described. Springer Verlag 2013-10 Article PeerReviewed Gillis, Richard B., Adams, Gary G., Heinze, Thomas, Nikolajski, Melanie, Harding, Stephen E. and Rowe, Arthur J. (2013) MultiSig: a new high-precision approach to the analysis of complex biomolecular systems. European Biophysics Journal, 42 (10). pp. 777-786. ISSN 0175-7571 http://link.springer.com/article/10.1007%2Fs00249-013-0924-y doi:10.1007/s00249-013-0924-y doi:10.1007/s00249-013-0924-y |
| spellingShingle | Gillis, Richard B. Adams, Gary G. Heinze, Thomas Nikolajski, Melanie Harding, Stephen E. Rowe, Arthur J. MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title | MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title_full | MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title_fullStr | MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title_full_unstemmed | MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title_short | MultiSig: a new high-precision approach to the analysis of complex biomolecular systems |
| title_sort | multisig: a new high-precision approach to the analysis of complex biomolecular systems |
| url | https://eprints.nottingham.ac.uk/3003/ https://eprints.nottingham.ac.uk/3003/ https://eprints.nottingham.ac.uk/3003/ |