A DEMETER-like DNA demethylase protein governs tomato fruit ripening
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental r...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
National Academy of Sciences
2015
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/29891/ |
| _version_ | 1848793873703239680 |
|---|---|
| author | Liu, Rui E. How-Kit, Alexandre Stammitti, Linda Teyssier, Emeline Rolin, Dominique Bertrand, Anne Halle, Stefanie Liu, Mingchun Kong, Junhua Wu, Chaoqun Degraeve-Guibault, Charlotte Chapman, Natalie Maucourt, Mickael Hodgman, Charlie Tost, Jörg Bouzayen, Mondher Hong, Yiguo Seymour, Graham B. Giovannoni, James Gallusci, Philippe |
| author_facet | Liu, Rui E. How-Kit, Alexandre Stammitti, Linda Teyssier, Emeline Rolin, Dominique Bertrand, Anne Halle, Stefanie Liu, Mingchun Kong, Junhua Wu, Chaoqun Degraeve-Guibault, Charlotte Chapman, Natalie Maucourt, Mickael Hodgman, Charlie Tost, Jörg Bouzayen, Mondher Hong, Yiguo Seymour, Graham B. Giovannoni, James Gallusci, Philippe |
| author_sort | Liu, Rui E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening; an important developmental process unique to plants. RNAi SlDML2 knock-down results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato |
| first_indexed | 2025-11-14T19:07:13Z |
| format | Article |
| id | nottingham-29891 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:07:13Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-298912020-05-04T20:07:53Z https://eprints.nottingham.ac.uk/29891/ A DEMETER-like DNA demethylase protein governs tomato fruit ripening Liu, Rui E. How-Kit, Alexandre Stammitti, Linda Teyssier, Emeline Rolin, Dominique Bertrand, Anne Halle, Stefanie Liu, Mingchun Kong, Junhua Wu, Chaoqun Degraeve-Guibault, Charlotte Chapman, Natalie Maucourt, Mickael Hodgman, Charlie Tost, Jörg Bouzayen, Mondher Hong, Yiguo Seymour, Graham B. Giovannoni, James Gallusci, Philippe In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening; an important developmental process unique to plants. RNAi SlDML2 knock-down results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato National Academy of Sciences 2015-08 Article PeerReviewed Liu, Rui E., How-Kit, Alexandre, Stammitti, Linda, Teyssier, Emeline, Rolin, Dominique, Bertrand, Anne, Halle, Stefanie, Liu, Mingchun, Kong, Junhua, Wu, Chaoqun, Degraeve-Guibault, Charlotte, Chapman, Natalie, Maucourt, Mickael, Hodgman, Charlie, Tost, Jörg, Bouzayen, Mondher, Hong, Yiguo, Seymour, Graham B., Giovannoni, James and Gallusci, Philippe (2015) A DEMETER-like DNA demethylase protein governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112 (34). pp. 10804-10809. ISSN 1091-6490 DNA methylation fruit ripening tomato Demeter like doi:10.1073/pnas.1503362112 doi:10.1073/pnas.1503362112 |
| spellingShingle | DNA methylation fruit ripening tomato Demeter like Liu, Rui E. How-Kit, Alexandre Stammitti, Linda Teyssier, Emeline Rolin, Dominique Bertrand, Anne Halle, Stefanie Liu, Mingchun Kong, Junhua Wu, Chaoqun Degraeve-Guibault, Charlotte Chapman, Natalie Maucourt, Mickael Hodgman, Charlie Tost, Jörg Bouzayen, Mondher Hong, Yiguo Seymour, Graham B. Giovannoni, James Gallusci, Philippe A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title | A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title_full | A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title_fullStr | A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title_full_unstemmed | A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title_short | A DEMETER-like DNA demethylase protein governs tomato fruit ripening |
| title_sort | demeter-like dna demethylase protein governs tomato fruit ripening |
| topic | DNA methylation fruit ripening tomato Demeter like |
| url | https://eprints.nottingham.ac.uk/29891/ https://eprints.nottingham.ac.uk/29891/ |