A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity
The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomy...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Genetics Society of America
2011
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| Online Access: | https://eprints.nottingham.ac.uk/2978/ |
| _version_ | 1848790922642325504 |
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| author | Brown, William R.A. Litti, Gianni Rosa, Carlos James, Steve Roberts, Ian Robert, Vincent Jolly, Neil Tang, Wen Baumann, Peter Green, Carter Schlegel, Kristina Young, Jonathan Hirchaud, Fabienne Leek, Spencer Thomas, Geraint Blomberg, Anders Warringer, Jonas |
| author_facet | Brown, William R.A. Litti, Gianni Rosa, Carlos James, Steve Roberts, Ian Robert, Vincent Jolly, Neil Tang, Wen Baumann, Peter Green, Carter Schlegel, Kristina Young, Jonathan Hirchaud, Fabienne Leek, Spencer Thomas, Geraint Blomberg, Anders Warringer, Jonas |
| author_sort | Brown, William R.A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell
biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent
natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces
pombe varies significantly in size but shows only limited variation in proliferation in different environments
compared with Saccharomyces cerevisiae. Nucleotide diversity, p, at a near neutral site, the central core of
the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed
karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis.
One translocation, found in 6 different isolates, including the type strain, has a geographically widespread
distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other
translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of
telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes. |
| first_indexed | 2025-11-14T18:20:19Z |
| format | Article |
| id | nottingham-2978 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:20:19Z |
| publishDate | 2011 |
| publisher | Genetics Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-29782020-05-04T16:31:31Z https://eprints.nottingham.ac.uk/2978/ A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity Brown, William R.A. Litti, Gianni Rosa, Carlos James, Steve Roberts, Ian Robert, Vincent Jolly, Neil Tang, Wen Baumann, Peter Green, Carter Schlegel, Kristina Young, Jonathan Hirchaud, Fabienne Leek, Spencer Thomas, Geraint Blomberg, Anders Warringer, Jonas The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces pombe varies significantly in size but shows only limited variation in proliferation in different environments compared with Saccharomyces cerevisiae. Nucleotide diversity, p, at a near neutral site, the central core of the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis. One translocation, found in 6 different isolates, including the type strain, has a geographically widespread distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes. Genetics Society of America 2011-12-01 Article PeerReviewed Brown, William R.A., Litti, Gianni, Rosa, Carlos, James, Steve, Roberts, Ian, Robert, Vincent, Jolly, Neil, Tang, Wen, Baumann, Peter, Green, Carter, Schlegel, Kristina, Young, Jonathan, Hirchaud, Fabienne, Leek, Spencer, Thomas, Geraint, Blomberg, Anders and Warringer, Jonas (2011) A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity. G3, 1 (7). pp. 615-626. ISSN 2160-1836 http://www.g3journal.org/content/1/7/615 doi:10.1534/g3.111.001123 doi:10.1534/g3.111.001123 |
| spellingShingle | Brown, William R.A. Litti, Gianni Rosa, Carlos James, Steve Roberts, Ian Robert, Vincent Jolly, Neil Tang, Wen Baumann, Peter Green, Carter Schlegel, Kristina Young, Jonathan Hirchaud, Fabienne Leek, Spencer Thomas, Geraint Blomberg, Anders Warringer, Jonas A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title | A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title_full | A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title_fullStr | A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title_full_unstemmed | A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title_short | A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| title_sort | geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity |
| url | https://eprints.nottingham.ac.uk/2978/ https://eprints.nottingham.ac.uk/2978/ https://eprints.nottingham.ac.uk/2978/ |