Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy
Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6...
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| Format: | Article |
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Royal Society of Chemistry
2014
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| Online Access: | https://eprints.nottingham.ac.uk/29756/ |
| _version_ | 1848793846876471296 |
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| author | Naemat, Abida Elsheikha, Hany M. Al-sandaqchib, Alaa Kong, Kenny Ghita, Adrian Notingher, Ioan |
| author_facet | Naemat, Abida Elsheikha, Hany M. Al-sandaqchib, Alaa Kong, Kenny Ghita, Adrian Notingher, Ioan |
| author_sort | Naemat, Abida |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 hr, 24 hr and 48 hr post-infection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time- and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuro-protective activity for eminent effects of changes in brain infection control practices. |
| first_indexed | 2025-11-14T19:06:47Z |
| format | Article |
| id | nottingham-29756 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:06:47Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-297562020-05-04T16:57:15Z https://eprints.nottingham.ac.uk/29756/ Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy Naemat, Abida Elsheikha, Hany M. Al-sandaqchib, Alaa Kong, Kenny Ghita, Adrian Notingher, Ioan Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 hr, 24 hr and 48 hr post-infection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time- and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuro-protective activity for eminent effects of changes in brain infection control practices. Royal Society of Chemistry 2014-11-19 Article PeerReviewed Naemat, Abida, Elsheikha, Hany M., Al-sandaqchib, Alaa, Kong, Kenny, Ghita, Adrian and Notingher, Ioan (2014) Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy. Analyst, 140 (3). pp. 756-764. ISSN 1364-5528 http://pubs.rsc.org/en/content/articlelanding/2015/an/c4an01810a#!divAbstract doi:10.1039/C4AN01810A doi:10.1039/C4AN01810A |
| spellingShingle | Naemat, Abida Elsheikha, Hany M. Al-sandaqchib, Alaa Kong, Kenny Ghita, Adrian Notingher, Ioan Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title | Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title_full | Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title_fullStr | Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title_full_unstemmed | Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title_short | Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman Spectroscopy |
| title_sort | analysis of interaction between the apicomplexan protozoan toxoplasma gondii and host cells using label-free raman spectroscopy |
| url | https://eprints.nottingham.ac.uk/29756/ https://eprints.nottingham.ac.uk/29756/ https://eprints.nottingham.ac.uk/29756/ |