Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene
The production of ornamentals is an important global industry, with Lilium being one of the six major bulb crops in the world. The international trade in ornamentals is in the order of £60-75 billion and is expected to increase worldwide by 2-4 % per annum. The continued success of the floriculture...
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Springer
2015
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| author | Núñez de Cáceres González, Francisco Davey, Michael R. Cancho Sánchez, Ester Wilson, Zoe A. |
| author_facet | Núñez de Cáceres González, Francisco Davey, Michael R. Cancho Sánchez, Ester Wilson, Zoe A. |
| author_sort | Núñez de Cáceres González, Francisco |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The production of ornamentals is an important global industry, with Lilium being one of the six major bulb crops in the world. The international trade in ornamentals is in the order of £60-75 billion and is expected to increase worldwide by 2-4 % per annum. The continued success of the floriculture industry depends on the introduction of new species/cultivars with major alterations in key agronomic characteristics, such as resistance to pathogens. Fungal diseases are the cause of reduced yields and marketable quality of cultivated plants, including ornamental species. The fungal pathogen Botrytis causes extreme economic losses to a wide range of crop species, including ornamentals such as Lilium. Agrobacterium-mediated transformation was used to develop Lilium oriental cv. ‘Star Gazer’ plants that ectopically overexpress the Rice Chitinase 10 gene (RCH10), under control of the CaMV35S promoter. Levels of conferred resistance linked to chitinase expression were evaluated by infection with Botrytis cinerea; sporulation was reduced in an in vitro assay and the relative expression of the RCH10 gene was determined by quantitative Reverse-Transcriptase PCR. The extent of resistance to Botrytis, compared to that of the wild type plants, showed a direct correlation with the level of chitinase gene expression. Transgenic plants grown to flowering showed no detrimental phenotypic effects associated with transgene expression. This is the first report of Lilium plants with resistance to Botrytis cinerea generated by a transgenic approach. |
| first_indexed | 2025-11-14T19:06:26Z |
| format | Article |
| id | nottingham-29661 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:06:26Z |
| publishDate | 2015 |
| publisher | Springer |
| recordtype | eprints |
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| spelling | nottingham-296612020-05-04T20:08:12Z https://eprints.nottingham.ac.uk/29661/ Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene Núñez de Cáceres González, Francisco Davey, Michael R. Cancho Sánchez, Ester Wilson, Zoe A. The production of ornamentals is an important global industry, with Lilium being one of the six major bulb crops in the world. The international trade in ornamentals is in the order of £60-75 billion and is expected to increase worldwide by 2-4 % per annum. The continued success of the floriculture industry depends on the introduction of new species/cultivars with major alterations in key agronomic characteristics, such as resistance to pathogens. Fungal diseases are the cause of reduced yields and marketable quality of cultivated plants, including ornamental species. The fungal pathogen Botrytis causes extreme economic losses to a wide range of crop species, including ornamentals such as Lilium. Agrobacterium-mediated transformation was used to develop Lilium oriental cv. ‘Star Gazer’ plants that ectopically overexpress the Rice Chitinase 10 gene (RCH10), under control of the CaMV35S promoter. Levels of conferred resistance linked to chitinase expression were evaluated by infection with Botrytis cinerea; sporulation was reduced in an in vitro assay and the relative expression of the RCH10 gene was determined by quantitative Reverse-Transcriptase PCR. The extent of resistance to Botrytis, compared to that of the wild type plants, showed a direct correlation with the level of chitinase gene expression. Transgenic plants grown to flowering showed no detrimental phenotypic effects associated with transgene expression. This is the first report of Lilium plants with resistance to Botrytis cinerea generated by a transgenic approach. Springer 2015-07 Article PeerReviewed Núñez de Cáceres González, Francisco, Davey, Michael R., Cancho Sánchez, Ester and Wilson, Zoe A. (2015) Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene. Plant Cell Reports, 34 (7). pp. 1201-1209. ISSN 1432-203X Lilium chitinase Agrobacterium Botrytis RCH10 ornamental http://link.springer.com/article/10.1007/s00299-015-1778-9?no-access=true doi:10.1007/s00299-015-1778-9 doi:10.1007/s00299-015-1778-9 |
| spellingShingle | Lilium chitinase Agrobacterium Botrytis RCH10 ornamental Núñez de Cáceres González, Francisco Davey, Michael R. Cancho Sánchez, Ester Wilson, Zoe A. Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title | Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title_full | Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title_fullStr | Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title_full_unstemmed | Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title_short | Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene |
| title_sort | conferred resistance to botrytis cinerea in lilium by overexpression of the rch10 chitinase gene |
| topic | Lilium chitinase Agrobacterium Botrytis RCH10 ornamental |
| url | https://eprints.nottingham.ac.uk/29661/ https://eprints.nottingham.ac.uk/29661/ https://eprints.nottingham.ac.uk/29661/ |