Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals
5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in...
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Landes Bioscience
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2961/ |
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| author | Almeida, Rimple D. Sottile, Virginie Loose, Matthew De Sousa, Paul A. Johnson, Andrew D. Ruzov, Alexey |
| author_facet | Almeida, Rimple D. Sottile, Virginie Loose, Matthew De Sousa, Paul A. Johnson, Andrew D. Ruzov, Alexey |
| author_sort | Almeida, Rimple D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | 5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in embryonic cell populations and in adult neuronal tissue. Here, we describe a novel method of semi-quantitative immunohistochemical detection of 5-hmC and utilize it to assess the levels of this modification in amphibian tissues. We show that, similar to mammalian embryos, 5-hmC is enriched in axolotl tadpoles compared with adult tissues. Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian skin and connective tissue in a mosaic manner. Our results illustrate that immunochemistry can be successfully used not only for spatial identification of cells enriched with 5-hmC, but also for the semi-quantitative assessment of the levels of this epigenetic modification in single cells of different tissues. |
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| format | Article |
| id | nottingham-2961 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:20:14Z |
| publishDate | 2012 |
| publisher | Landes Bioscience |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-29612020-05-04T16:32:03Z https://eprints.nottingham.ac.uk/2961/ Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals Almeida, Rimple D. Sottile, Virginie Loose, Matthew De Sousa, Paul A. Johnson, Andrew D. Ruzov, Alexey 5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in embryonic cell populations and in adult neuronal tissue. Here, we describe a novel method of semi-quantitative immunohistochemical detection of 5-hmC and utilize it to assess the levels of this modification in amphibian tissues. We show that, similar to mammalian embryos, 5-hmC is enriched in axolotl tadpoles compared with adult tissues. Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian skin and connective tissue in a mosaic manner. Our results illustrate that immunochemistry can be successfully used not only for spatial identification of cells enriched with 5-hmC, but also for the semi-quantitative assessment of the levels of this epigenetic modification in single cells of different tissues. Landes Bioscience 2012-02-01 Article PeerReviewed Almeida, Rimple D., Sottile, Virginie, Loose, Matthew, De Sousa, Paul A., Johnson, Andrew D. and Ruzov, Alexey (2012) Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals. Epigenetics, 7 (2). pp. 137-140. ISSN 1559-2294 https://www.landesbioscience.com/journals/epigenetics/article/18949/ doi:10.4161/epi.7.2.18949 doi:10.4161/epi.7.2.18949 |
| spellingShingle | Almeida, Rimple D. Sottile, Virginie Loose, Matthew De Sousa, Paul A. Johnson, Andrew D. Ruzov, Alexey Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title | Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title_full | Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title_fullStr | Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title_full_unstemmed | Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title_short | Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| title_sort | semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals |
| url | https://eprints.nottingham.ac.uk/2961/ https://eprints.nottingham.ac.uk/2961/ https://eprints.nottingham.ac.uk/2961/ |