Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes
Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extrac...
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Cambridge University Press
2007
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| Online Access: | https://eprints.nottingham.ac.uk/29417/ |
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| author | Stepek, Gillian Curtis, R.H.C. Kerry, B.R. Shewry, P.R. Clark, S.J. Lowe, Ann Duce, I.R. Buttle, David J. Behnke, J.M. |
| author_facet | Stepek, Gillian Curtis, R.H.C. Kerry, B.R. Shewry, P.R. Clark, S.J. Lowe, Ann Duce, I.R. Buttle, David J. Behnke, J.M. |
| author_sort | Stepek, Gillian |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants. |
| first_indexed | 2025-11-14T19:05:46Z |
| format | Article |
| id | nottingham-29417 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:05:46Z |
| publishDate | 2007 |
| publisher | Cambridge University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-294172020-05-04T20:29:13Z https://eprints.nottingham.ac.uk/29417/ Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes Stepek, Gillian Curtis, R.H.C. Kerry, B.R. Shewry, P.R. Clark, S.J. Lowe, Ann Duce, I.R. Buttle, David J. Behnke, J.M. Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants. Cambridge University Press 2007 Article PeerReviewed Stepek, Gillian, Curtis, R.H.C., Kerry, B.R., Shewry, P.R., Clark, S.J., Lowe, Ann, Duce, I.R., Buttle, David J. and Behnke, J.M. (2007) Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes. Parasitology, 134 (12). pp. 1831-1838. ISSN 0031-1820 plant parasitic nematodes Meloidogyne spp. Globodera rostochiensis plant cysteine proteinases in vitro nematicide http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1367720&fileId=S0031182007003289 doi:10.1017/S0031182007003289 doi:10.1017/S0031182007003289 |
| spellingShingle | plant parasitic nematodes Meloidogyne spp. Globodera rostochiensis plant cysteine proteinases in vitro nematicide Stepek, Gillian Curtis, R.H.C. Kerry, B.R. Shewry, P.R. Clark, S.J. Lowe, Ann Duce, I.R. Buttle, David J. Behnke, J.M. Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title | Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title_full | Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title_fullStr | Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title_full_unstemmed | Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title_short | Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| title_sort | nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes |
| topic | plant parasitic nematodes Meloidogyne spp. Globodera rostochiensis plant cysteine proteinases in vitro nematicide |
| url | https://eprints.nottingham.ac.uk/29417/ https://eprints.nottingham.ac.uk/29417/ https://eprints.nottingham.ac.uk/29417/ |