Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo
Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, Heligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris mu...
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Cambridge University Press
2007
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| Online Access: | https://eprints.nottingham.ac.uk/29413/ |
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| author | Stepek, Gillian Lowe, Ann Buttle, David J. Duce, I.R. Behnke, Jerzy M. |
| author_facet | Stepek, Gillian Lowe, Ann Buttle, David J. Duce, I.R. Behnke, Jerzy M. |
| author_sort | Stepek, Gillian |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, Heligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris muris (which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. In this paper, we describe the in vitro and in vivo effects of these plant extracts against the rodent nematode, Protospirura muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematode in vitro. In vivo, in contrast to the efficacy against H. polygyrus and T. muris, papaya latex only showed efficacy against P. muricola adult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract. |
| first_indexed | 2025-11-14T19:05:45Z |
| format | Article |
| id | nottingham-29413 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:05:45Z |
| publishDate | 2007 |
| publisher | Cambridge University Press |
| recordtype | eprints |
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| spelling | nottingham-294132020-05-04T20:28:33Z https://eprints.nottingham.ac.uk/29413/ Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo Stepek, Gillian Lowe, Ann Buttle, David J. Duce, I.R. Behnke, Jerzy M. Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, Heligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris muris (which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. In this paper, we describe the in vitro and in vivo effects of these plant extracts against the rodent nematode, Protospirura muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematode in vitro. In vivo, in contrast to the efficacy against H. polygyrus and T. muris, papaya latex only showed efficacy against P. muricola adult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract. Cambridge University Press 2007-01 Article PeerReviewed Stepek, Gillian, Lowe, Ann, Buttle, David J., Duce, I.R. and Behnke, Jerzy M. (2007) Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo. Parasitology, 134 (1). pp. 103-112. ISSN 0031-1820 Protospirura muricola plant cysteine proteinases papaya pineapple fig in vitro in vivo antacid anthelmintic gastrointestinal nematodes INTRODUCTION http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=562296&fileId=S0031182006001302 doi:10.1017/S0031182006001302 doi:10.1017/S0031182006001302 |
| spellingShingle | Protospirura muricola plant cysteine proteinases papaya pineapple fig in vitro in vivo antacid anthelmintic gastrointestinal nematodes INTRODUCTION Stepek, Gillian Lowe, Ann Buttle, David J. Duce, I.R. Behnke, Jerzy M. Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo |
| title | Anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, Protospirura muricola, in vitro
and in vivo |
| title_full | Anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, Protospirura muricola, in vitro
and in vivo |
| title_fullStr | Anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, Protospirura muricola, in vitro
and in vivo |
| title_full_unstemmed | Anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, Protospirura muricola, in vitro
and in vivo |
| title_short | Anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, Protospirura muricola, in vitro
and in vivo |
| title_sort | anthelmintic action of plant cysteine proteinases against
the rodent stomach nematode, protospirura muricola, in vitro
and in vivo |
| topic | Protospirura muricola plant cysteine proteinases papaya pineapple fig in vitro in vivo antacid anthelmintic gastrointestinal nematodes INTRODUCTION |
| url | https://eprints.nottingham.ac.uk/29413/ https://eprints.nottingham.ac.uk/29413/ https://eprints.nottingham.ac.uk/29413/ |