Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species

The gastro-intestinal (GI) nematode Heligmosomoides polygyrus is an important experimental model in laboratory mice and a well-studied parasite of wood mice in the field. Despite an extensive literature, the taxonomy of this parasite in different hosts is confused, and it is unclear whether laborato...

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Main Authors:	Cable, J., Harris, P.D., Lewis, J.W., Behnke, J.M.
Format:	Article
Published:	Cambridge University Press 2006
Subjects:	Heligmosomatid Apodemus sylvaticus GI nematodes
Online Access:	https://eprints.nottingham.ac.uk/29411/

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author	Cable, J. Harris, P.D. Lewis, J.W. Behnke, J.M.
author_facet	Cable, J. Harris, P.D. Lewis, J.W. Behnke, J.M.
author_sort	Cable, J.
building	Nottingham Research Data Repository
collection	Online Access
description	The gastro-intestinal (GI) nematode Heligmosomoides polygyrus is an important experimental model in laboratory mice and a well-studied parasite of wood mice in the field. Despite an extensive literature, the taxonomy of this parasite in different hosts is confused, and it is unclear whether laboratory and field systems represent the same or different Operational Taxonomic Units (OTUs). Molecular analyses reveal high sequence divergence between H. p. bakeri (laboratory) and H. p. polygyrus (field); 3% difference in the ribosomal DNA Internal Transcribed Spacers (ITS) and 8.6% variation in the more rapidly evolving mitochondrial cytochrome c oxidase I (COI) gene. The COI sequence of U.K. H. p. polygyrus is more similar to H. glareoli from voles than to H. p. bakeri, while a single isolate of H. p. polygyrus from Guernsey confirms the extent of genetic variation between H. p. polygyrus populations. Analysis of molecular variance demonstrated that mtCOI sequence variation is associated primarily with groups with distinct ITS2 sequences, and with host identity, but is not partitioned significantly with a single combined taxon H. polygyrus incorporating European and North American isolates. We conclude therefore that the laboratory OTUshould be raised to the level of a distinct species, as H. bakeri from the laboratory mouse Mus musculus, and we reject the hypothesis that H. bakeri has diverged from H. polygyrus in the recent past following introduction into America. However, we are unable to reject the hypothesis that H. polygyrus and H. bakeri are sister taxa, and it may be that H. polygyrus is polyphyletic or paraphyletic.
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format	Article
id	nottingham-29411
institution	University of Nottingham Malaysia Campus
institution_category	Local University
last_indexed	2025-11-14T19:05:44Z
publishDate	2006
publisher	Cambridge University Press
recordtype	eprints
repository_type	Digital Repository
spelling	nottingham-294112020-05-04T20:29:46Z https://eprints.nottingham.ac.uk/29411/ Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species Cable, J. Harris, P.D. Lewis, J.W. Behnke, J.M. The gastro-intestinal (GI) nematode Heligmosomoides polygyrus is an important experimental model in laboratory mice and a well-studied parasite of wood mice in the field. Despite an extensive literature, the taxonomy of this parasite in different hosts is confused, and it is unclear whether laboratory and field systems represent the same or different Operational Taxonomic Units (OTUs). Molecular analyses reveal high sequence divergence between H. p. bakeri (laboratory) and H. p. polygyrus (field); 3% difference in the ribosomal DNA Internal Transcribed Spacers (ITS) and 8.6% variation in the more rapidly evolving mitochondrial cytochrome c oxidase I (COI) gene. The COI sequence of U.K. H. p. polygyrus is more similar to H. glareoli from voles than to H. p. bakeri, while a single isolate of H. p. polygyrus from Guernsey confirms the extent of genetic variation between H. p. polygyrus populations. Analysis of molecular variance demonstrated that mtCOI sequence variation is associated primarily with groups with distinct ITS2 sequences, and with host identity, but is not partitioned significantly with a single combined taxon H. polygyrus incorporating European and North American isolates. We conclude therefore that the laboratory OTUshould be raised to the level of a distinct species, as H. bakeri from the laboratory mouse Mus musculus, and we reject the hypothesis that H. bakeri has diverged from H. polygyrus in the recent past following introduction into America. However, we are unable to reject the hypothesis that H. polygyrus and H. bakeri are sister taxa, and it may be that H. polygyrus is polyphyletic or paraphyletic. Cambridge University Press 2006 Article PeerReviewed Cable, J., Harris, P.D., Lewis, J.W. and Behnke, J.M. (2006) Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species. Parasitology, 133 (1). pp. 111-122. ISSN 0031-1820 Heligmosomatid Apodemus sylvaticus GI nematodes http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=445998&fileId=S0031182006000047 doi:10.1017/S0031182006000047 doi:10.1017/S0031182006000047
spellingShingle	Heligmosomatid Apodemus sylvaticus GI nematodes Cable, J. Harris, P.D. Lewis, J.W. Behnke, J.M. Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title	Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title_full	Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title_fullStr	Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title_full_unstemmed	Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title_short	Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species
title_sort	molecular evidence that heligmosomoides polygyrus from laboratory mice and wood mice are separate species
topic	Heligmosomatid Apodemus sylvaticus GI nematodes
url	https://eprints.nottingham.ac.uk/29411/ https://eprints.nottingham.ac.uk/29411/ https://eprints.nottingham.ac.uk/29411/

Molecular evidence that Heligmosomoides polygyrus from laboratory mice and wood mice are separate species

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