Sequential induction of auxin efflux and influx carriers regulates lateral root emergence
In Arabidopsis, lateral roots originate from pericycle cells deep within the primary root. New lateral root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards the outer tissues where it triggers cell separation by...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Nature Publishing Group
2013
|
| Online Access: | https://eprints.nottingham.ac.uk/2941/ |
| _version_ | 1848790914218065920 |
|---|---|
| author | Peret, Benjamin Middleton, Alistair M. French, Andrew P. Larrieu, Antoine Bishopp, Antony Njo, Maria Wells, Darren M. Porco, Silvana Mellor, Nathan. Band, Leah R. Casimiro, Ilda Kleine-Vehn, Jurgen Vanneste, Steffen Sairanen, Ilkka Mallet, Romain Sandberg, Goran Ljung, Karin Beeckman, Tom Benkova, Eva Friml, Jiri Kramer, Eric King, John R. De Smet, Ive Pridmore, Tony Owen, Markus R. Bennett, Malcolm J. |
| author_facet | Peret, Benjamin Middleton, Alistair M. French, Andrew P. Larrieu, Antoine Bishopp, Antony Njo, Maria Wells, Darren M. Porco, Silvana Mellor, Nathan. Band, Leah R. Casimiro, Ilda Kleine-Vehn, Jurgen Vanneste, Steffen Sairanen, Ilkka Mallet, Romain Sandberg, Goran Ljung, Karin Beeckman, Tom Benkova, Eva Friml, Jiri Kramer, Eric King, John R. De Smet, Ive Pridmore, Tony Owen, Markus R. Bennett, Malcolm J. |
| author_sort | Peret, Benjamin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In Arabidopsis, lateral roots originate from pericycle cells deep within the primary root. New lateral root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards the outer tissues where it triggers cell separation by inducing both the auxin influx carrier LAX3 and cell‐wall enzymes. LAX3 is expressed in just two cell files overlaying new LRP. To understand how this striking pattern of LAX3 expression is regulated, we developed a mathematical model that captures the network regulating its expression and auxin transport within realistic three‐dimensional cell and tissue geometries. Our model revealed that, for the LAX3 spatial expression to be robust to natural variations in root tissue geometry, an efflux carrier is required—later identified to be PIN3. To prevent LAX3 from being transiently expressed in multiple cell files, PIN3 and LAX3 must be induced consecutively, which we later demonstrated to be the case. Our study exemplifies how mathematical models can be used to direct experiments to elucidate complex developmental processes. |
| first_indexed | 2025-11-14T18:20:11Z |
| format | Article |
| id | nottingham-2941 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:20:11Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-29412020-05-04T16:39:16Z https://eprints.nottingham.ac.uk/2941/ Sequential induction of auxin efflux and influx carriers regulates lateral root emergence Peret, Benjamin Middleton, Alistair M. French, Andrew P. Larrieu, Antoine Bishopp, Antony Njo, Maria Wells, Darren M. Porco, Silvana Mellor, Nathan. Band, Leah R. Casimiro, Ilda Kleine-Vehn, Jurgen Vanneste, Steffen Sairanen, Ilkka Mallet, Romain Sandberg, Goran Ljung, Karin Beeckman, Tom Benkova, Eva Friml, Jiri Kramer, Eric King, John R. De Smet, Ive Pridmore, Tony Owen, Markus R. Bennett, Malcolm J. In Arabidopsis, lateral roots originate from pericycle cells deep within the primary root. New lateral root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards the outer tissues where it triggers cell separation by inducing both the auxin influx carrier LAX3 and cell‐wall enzymes. LAX3 is expressed in just two cell files overlaying new LRP. To understand how this striking pattern of LAX3 expression is regulated, we developed a mathematical model that captures the network regulating its expression and auxin transport within realistic three‐dimensional cell and tissue geometries. Our model revealed that, for the LAX3 spatial expression to be robust to natural variations in root tissue geometry, an efflux carrier is required—later identified to be PIN3. To prevent LAX3 from being transiently expressed in multiple cell files, PIN3 and LAX3 must be induced consecutively, which we later demonstrated to be the case. Our study exemplifies how mathematical models can be used to direct experiments to elucidate complex developmental processes. Nature Publishing Group 2013-10-22 Article PeerReviewed Peret, Benjamin, Middleton, Alistair M., French, Andrew P., Larrieu, Antoine, Bishopp, Antony, Njo, Maria, Wells, Darren M., Porco, Silvana, Mellor, Nathan., Band, Leah R., Casimiro, Ilda, Kleine-Vehn, Jurgen, Vanneste, Steffen, Sairanen, Ilkka, Mallet, Romain, Sandberg, Goran, Ljung, Karin, Beeckman, Tom, Benkova, Eva, Friml, Jiri, Kramer, Eric, King, John R., De Smet, Ive, Pridmore, Tony, Owen, Markus R. and Bennett, Malcolm J. (2013) Sequential induction of auxin efflux and influx carriers regulates lateral root emergence. Molecular Systems Biology, 9 (1). 699/1-699/15. ISSN 1744-4292 http://msb.embopress.org/content/9/1/699 doi:10.1038/msb.2013.43 doi:10.1038/msb.2013.43 |
| spellingShingle | Peret, Benjamin Middleton, Alistair M. French, Andrew P. Larrieu, Antoine Bishopp, Antony Njo, Maria Wells, Darren M. Porco, Silvana Mellor, Nathan. Band, Leah R. Casimiro, Ilda Kleine-Vehn, Jurgen Vanneste, Steffen Sairanen, Ilkka Mallet, Romain Sandberg, Goran Ljung, Karin Beeckman, Tom Benkova, Eva Friml, Jiri Kramer, Eric King, John R. De Smet, Ive Pridmore, Tony Owen, Markus R. Bennett, Malcolm J. Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title | Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title_full | Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title_fullStr | Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title_full_unstemmed | Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title_short | Sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| title_sort | sequential induction of auxin efflux and influx carriers regulates lateral root emergence |
| url | https://eprints.nottingham.ac.uk/2941/ https://eprints.nottingham.ac.uk/2941/ https://eprints.nottingham.ac.uk/2941/ |